Lariviere William R, Mogil Jeffrey S
Departments of Anesthesiology and Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Methods Mol Biol. 2010;617:261-78. doi: 10.1007/978-1-60327-323-7_20.
Pain and analgesia traits are heritable in humans and in mice. To better understand the mechanisms of heritability, animal models that provide greater control than is possible in humans over genotype, previous history, environment, and stimulus parameters are available. This chapter will highlight several common methods to study the genetic mechanisms of heritable sensitivity to pain and pain-related traits in rodents. Methods to demonstrate and estimate the heritability of a trait are discussed, as are genetic correlation analysis and linkage mapping. Practical concerns are highlighted throughout this chapter. Due to limitations on the use of humans for similarly powered experiments, these and other animal models remain an essential component in the study of heritable mechanisms of pain and analgesia.
疼痛和镇痛特性在人类和小鼠中具有遗传性。为了更好地理解遗传机制，有一些动物模型可供使用，这些模型在基因型、既往史、环境和刺激参数方面比人类实验能提供更好的控制。本章将重点介绍几种研究啮齿动物对疼痛和疼痛相关特性遗传性敏感的遗传机制的常用方法。文中讨论了证明和估计性状遗传性的方法，以及遗传相关性分析和连锁图谱分析。本章自始至终都强调了实际问题。由于在人类中进行类似规模实验存在限制，这些以及其他动物模型仍然是疼痛和镇痛遗传机制研究的重要组成部分。
