Division of Nuclear Chemistry, Department of Chemistry, Faculty of Science, Ege University, Bornova, 35100 Izmir, Turkey.
Macromol Biosci. 2010 Jun 11;10(6):657-63. doi: 10.1002/mabi.201000009.
Poly(vinyl alcohol)-pyrene-anti-metadherin (PVA-Py-(Anti-MTDH)), a novel antibody based water soluble probe containing both fluorescent and target sites in the structure for in vitro imaging of breast cancer cells is reported here. Since breast cancer cells have an excess of MDTH protein expressed on the surface, a PVA-Py prepared by "Click chemistry" approach is targeted by Anti-MTDH antibody and applied to the MCF-7 cell line. After characterization, the designed architecture was evaluated in terms of cell incorporation efficiency and compared with a non-targeted structure (PVA-Py). Atomic force microscopy (AFM) and fluorescence microscopy images of cells after incubation of the probe molecules were also obtained to monitor the interaction of the probes with the cancerous cells.
聚（聚乙烯醇）-芘-抗 METADHERIN（PVA-Py-（Anti-MTDH）），一种新型抗体的基于水溶性的探针，包含结构中的荧光基团和靶点，用于乳腺癌细胞的体外成像。由于乳腺癌细胞表面表达过量的 MDTH 蛋白，因此通过“点击化学”方法制备的 PVA-Py 被 Anti-MTDH 抗体靶向，并应用于 MCF-7 细胞系。经过表征，根据细胞掺入效率对设计的结构进行了评估，并与非靶向结构（PVA-Py）进行了比较。还通过原子力显微镜（AFM）和荧光显微镜获得了探针分子孵育后细胞的图像，以监测探针与癌细胞的相互作用。
