School of Life Science and Technology, Tongji University, Shanghai 200092, People's Republic of China.
Appl Biochem Biotechnol. 2011 Apr;163(7):813-25. doi: 10.1007/s12010-010-9085-y. Epub 2010 Oct 16.
Silica-coated magnetic polystyrene nanospheres (MPN) containing CdTe/CdS quantum dots (QDs) and Fe₃O₄ nanoparticles were prepared, and novel anti-EGFR antibodies were conjugated onto these magneto-fluorescent nanocomposites (MPN-QDs-SiO₂) for cancer cell targeting, imaging and collection. Transmission electron microscopy (TEM), scanning electron microscopy (SEM) images and energy-dispersive x-ray spectrometry (EDS) data showed that the MPN had been successfully coated with QDs and a silica shell, and the nanocomposites obtained with negative charged surfaces were well dispersed. The bioconjugates could be used for specifically labeling and separating cancer cells (MDA-MB-435S, SMMC-7721), but did not recognize and separate the K562 cells because the human epidermal growth factor receptor (EGFR) was not expressed on the surface. Because the anti-EGFR antibody, which we have developed, could specifically recognize certain cancer cells that highly expressed EGFR on their surface, these nanoscale bioconjugates, synchronously exhibiting fluorescence and magnetism, may be used in novel bioprobes for labeling and collecting rare cancer cells, which may be beneficial for early cancer diagnosis.
硅烷化磁性聚苯乙烯纳米球（MPN）表面包覆有碲化镉/硫化镉量子点（QDs）和四氧化三铁纳米颗粒，新型抗表皮生长因子受体（EGFR）抗体被偶联到这些磁荧光纳米复合材料（MPN-QDs-SiO₂）上，用于靶向、成像和收集癌细胞。透射电子显微镜（TEM）、扫描电子显微镜（SEM）图像和能谱分析（EDS）数据表明，MPN 已成功包覆 QDs 和二氧化硅壳，得到的带负电荷的纳米复合材料分散性良好。这些生物缀合物可用于特异性标记和分离癌细胞（MDA-MB-435S、SMMC-7721），但不能识别和分离 K562 细胞，因为细胞表面不表达人表皮生长因子受体（EGFR）。因为我们开发的抗 EGFR 抗体可以特异性识别表面高度表达 EGFR 的特定癌细胞，这些同时具有荧光和磁性的纳米级生物缀合物，可能被用于新型生物探针来标记和收集罕见的癌细胞，这对于早期癌症诊断可能是有益的。
