Hun Xu, Zhang Zhujun
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2009 Oct 1;74(2):410-4. doi: 10.1016/j.saa.2009.06.033. Epub 2009 Jun 18.
Fluorescent nanoparticles (FNs) with unique optical properties may be useful as biosensors in living cancer cell imaging and cancer targeting. In this study, anti-EGFR antibody conjugated fluorescent nanoparticles (FNs) (anti-EGFR antibody conjugated FNs) probe was used to detect breast cancer cells. FNs with excellent character such as non-toxicity and photostability were first synthesized with a simple, cost-effective and environmentally friendly modified Stober synthesis method, and then successfully modified with anti-EGFR antibody. This kind of fluorescence probe based on the anti-EGFR antibody conjugated FNs has been used to detect breast cancer cells with fluorescence microscopy imaging technology. The experimental results demonstrate that the anti-EGFR antibody conjugated FNs can effectively recognize breast cancer cells and exhibited good sensitivity and exceptional photostability, which would provide a novel way for the diagnosis and curative effect observation of breast cancer cells and offer a new method in detecting EGFR.
具有独特光学性质的荧光纳米颗粒（FNs）在活癌细胞成像和癌症靶向中可用作生物传感器。在本研究中，抗表皮生长因子受体（EGFR）抗体偶联荧光纳米颗粒（FNs）（抗EGFR抗体偶联FNs）探针被用于检测乳腺癌细胞。首先采用一种简单、经济高效且环保的改良施托伯合成方法合成了具有无毒、光稳定性等优异特性的FNs，然后成功地用抗EGFR抗体进行了修饰。这种基于抗EGFR抗体偶联FNs的荧光探针已通过荧光显微镜成像技术用于检测乳腺癌细胞。实验结果表明，抗EGFR抗体偶联FNs能够有效识别乳腺癌细胞，并表现出良好的灵敏度和出色的光稳定性，这将为乳腺癌细胞的诊断和疗效观察提供一种新方法，并为检测EGFR提供一种新手段。
