School of Mechanical Engineering, Korea University, Seoul, Korea.
Ann Biomed Eng. 2010 Mar;38(3):1164-77. doi: 10.1007/s10439-010-9899-3.
Recent advances in microfluidic technologies have opened the door for creating more realistic in vitro cell culture methods that replicate many aspects of the true in vivo microenvironment. These new designs (i) provide enormous flexibility in controlling the critical biochemical and biomechanical factors that influence cell behavior, (ii) allow for the introduction of multiple cell types in a single system, (iii) provide for the establishment of biochemical gradients in two- or three-dimensional geometries, and (iv) allow for high quality, time-lapse imaging. Here, some of the recent developments are reviewed, with a focus on studies from our own laboratory in three separate areas: angiogenesis, cell migration in the context of tumor cell-endothelial interactions, and liver tissue engineering.
微流控技术的最新进展为创建更逼真的体外细胞培养方法打开了大门，这些方法复制了真实体内微环境的许多方面。这些新设计（i）在控制影响细胞行为的关键生化和生物力学因素方面提供了巨大的灵活性，（ii）允许在单个系统中引入多种细胞类型，（iii）在二维或三维几何形状中建立生化梯度，（iv）允许进行高质量、延时成像。在这里，我们回顾了一些最新的进展，并重点介绍了我们自己实验室在三个不同领域的研究：血管生成、肿瘤细胞-内皮细胞相互作用背景下的细胞迁移，以及肝脏组织工程。
