Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
FEMS Microbiol Lett. 2010 May;306(1):15-21. doi: 10.1111/j.1574-6968.2010.01917.x. Epub 2010 Jan 29.
Escherichia coli isolates from diseased pigs were examined for antimicrobial susceptibility to 12 antimicrobials and possession of virulence genes (VGs), and then grouped according to the phylogenetic background and genetic relatedness. Associations between antimicrobial resistance (AMR) and VGs and between AMR and phylogenetic group were subsequently assessed. The results showed that most isolates (91%) were epidemiologically unrelated. Multiple antimicrobial-resistant phenotypes (>or=5 antimicrobials) were observed in 89% of E. coli strains and the most frequent types of resistance were to sulfamethoxazole (95%), tetracycline (94%), chloramphenicol (89%), and streptomycin (84%). The majority of isolates belonged to phylogenetic group A (84%). The most prevalent VG was EAST1 (64%), followed by Stx2e (63%) and eae (47%). Resistance to ceftiofur was associated with the presence of certain VGs, whereas resistance to doxycycline and kanamycin was associated with the absence of certain VGs. These findings suggest that multidrug resistance phenotypes, a variety of VGs, and the clear associations between resistance and VGs are commonly present in E. coli strains from diseased pigs. These results indicate that there is a great need for surveillance programs in China to monitor AMR in pathogenic E. coli strains.
从患病猪中分离的大肠杆菌被检测对 12 种抗菌药物的药敏性和毒力基因（VGs）的携带情况，然后根据系统发育背景和遗传相关性进行分组。随后评估了 AMR 与 VGs 之间以及 AMR 与系统发育群之间的相关性。结果表明，大多数分离株（91%）在流行病学上无关。90%的大肠杆菌菌株表现出多种抗微生物药物耐药表型（≥5 种抗菌药物），最常见的耐药类型为磺胺甲恶唑（95%）、四环素（94%）、氯霉素（89%）和链霉素（84%）。大多数分离株属于 A 群（84%）。最常见的 VG 是 EAST1（64%），其次是 Stx2e（63%）和 eae（47%）。对头孢噻呋的耐药性与某些 VGs 的存在有关，而对强力霉素和卡那霉素的耐药性与某些 VGs 的缺失有关。这些发现表明，多药耐药表型、多种 VGs 以及耐药性与 VGs 之间的明确相关性在患病猪的大肠杆菌菌株中普遍存在。这些结果表明，中国非常有必要开展监测项目，以监测致病性大肠杆菌菌株中的 AMR。
