Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong-dong, Jeongeup, Republic of Korea.
Chem Biol Interact. 2010 Jun 7;186(1):90-5. doi: 10.1016/j.cbi.2010.03.032. Epub 2010 Mar 23.
Silk fibers have proven to be effective in many clinical applications as well as for clothing. In addition to the substantial effect of silk fibers, the present study was conducted to explore its importance in a new dimension to reinforce the effects of its physiological function regarding anti-tumor activity and immune response with gamma-irradiated silk fibroin (GISF). The cytotoxicity results showed that pre-treatment of GISF in the mouse peritoneal macrophages (MPM) indicated a higher proliferative effect than that of non-irradiated silk fibroin (NISF) in a concentration-dependent manner. Based on the cytotoxicity result of MPM, GISF (50 and 150 kGy) was selected for an ex vivo study in an animal (C57BL6) system and evaluated about whether the non-specific immune response was also related to GISF. GISF (50 and 150 kGy) augmented immune responsiveness via activation of NK cells, T lymphocytes proliferation, NO production, and cytokine level, such as IL-6, IL-2, IL-12, IFN-gamma, TNF-alpha, as compared with NISF, which strongly suggested that GISF significantly augmented an important element of all aspects of the innate and adaptive immune system. Therefore, from these results, it seems likely that the GISF will play a potent role in eliciting the effect of the non-specific immune response and anti-tumor activity as a value-added product in the medical industry.
蚕丝纤维已被证明在许多临床应用以及服装方面都非常有效。除了蚕丝纤维的显著作用外，本研究还旨在探索其在新维度中的重要性，以增强其抗肿瘤活性和免疫反应的生理功能，用γ辐照丝素蛋白（GISF）加以强化。细胞毒性结果表明，在小鼠腹腔巨噬细胞（MPM）中预先处理 GISF 比非辐照丝素蛋白（NISF）具有更高的增殖作用，呈浓度依赖性。基于 MPM 的细胞毒性结果，选择 GISF（50 和 150 kGy）进行动物（C57BL6）系统的离体研究，并评估非特异性免疫反应是否也与 GISF 相关。与 NISF 相比，GISF（50 和 150 kGy）通过激活 NK 细胞、T 淋巴细胞增殖、NO 产生和细胞因子水平（如 IL-6、IL-2、IL-12、IFN-γ、TNF-α）增强免疫反应，这强烈表明 GISF 显著增强了固有和适应性免疫系统所有方面的一个重要因素。因此，从这些结果来看，GISF 似乎很可能在引发非特异性免疫反应和抗肿瘤活性方面发挥强大作用，成为医疗行业的增值产品。
