Department of Cell and Molecular Biology and Pathogenic Bioagents, Faculdade de Medicina de Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
PLoS One. 2010 Mar 22;5(3):e9776. doi: 10.1371/journal.pone.0009776.
The D-mannose binding lectin ArtinM is known to recruit neutrophils, to degranulate mast cells and may have potential therapeutic applications. However, the effect of ArtinM on mast cell recruitment has not been investigated.
Male Wistar rats were injected i.p. with ArtinM or ConA (control). The ability of the lectin to degranulate peritoneal and mesenteric mast cells was examined. Recruitment of mast cells to the peritoneal cavity and mesentery after ArtinM injection was examined with or without depletion of peritoneal mast cells by distilled water.
ArtinM degranulated both peritoneal and mesentery mast cells in vitro. Three days after i.p. injection of the lectin there were reduced numbers of mast cells in the peritoneal lavage, while at 7 days post injection of ArtinM, the number of peritoneal mast cells was close to control values. Since immature mast cells are recruited from the bone marrow, the effect of the lectin on bone marrow mast cells was examined. Injection of ArtinM resulted in an increased number of mast cells in the bone marrow. To determine if degranulation of mast cells in the peritoneal cavity was required for the increase in bone marrow mast cells, the peritoneal cavity was depleted of mast cells with ultrapure water. Exposure to ArtinM increased the number of mast cells in the bone marrow of rats depleted of peritoneal mast cells.
The ArtinM induced recruitment of mast cells from the bone marrow to the peritoneal cavity may partially explain the therapeutic actions of ArtinM.
已知 D-甘露糖结合凝集素 ArtinM 可募集中性粒细胞、脱颗粒肥大细胞，并有潜在的治疗应用。然而，ArtinM 对肥大细胞募集的影响尚未被研究过。
雄性 Wistar 大鼠经腹腔内注射 ArtinM 或 ConA（对照）。研究了凝集素体外脱颗粒腹膜和肠系膜肥大细胞的能力。在没有用蒸馏水耗尽腹膜肥大细胞的情况下，观察 ArtinM 注射后对腹腔和肠系膜肥大细胞的募集作用。
ArtinM 在体外脱颗粒腹膜和肠系膜肥大细胞。在注射凝集素 3 天后，腹膜灌洗液中的肥大细胞数量减少，而在注射 ArtinM 7 天后，腹膜肥大细胞的数量接近对照值。由于成熟的肥大细胞是从骨髓招募而来的，因此研究了凝集素对骨髓肥大细胞的影响。注射 ArtinM 导致骨髓中的肥大细胞数量增加。为了确定腹腔内肥大细胞脱颗粒是否是骨髓肥大细胞增加所必需的，用超纯水耗尽腹腔内的肥大细胞。暴露于 ArtinM 增加了耗尽腹膜肥大细胞的大鼠骨髓中肥大细胞的数量。
ArtinM 诱导从骨髓招募肥大细胞到腹腔可能部分解释了 ArtinM 的治疗作用。
