Department of Biological Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo, 112-8610, Japan.
J Plant Res. 2010 Nov;123(6):801-5. doi: 10.1007/s10265-010-0325-6. Epub 2010 Mar 26.
In the genome of the model legume Lotus japonicus, dihydroflavonol 4-reductase (DFR), which is the first committed enzyme of the anthocyanin and proanthocyanidin (PA) pathways, is encoded as a tandemly arrayed five-gene family. Expression analysis revealed that both organ specificity and stress responsiveness differ among the DFRs. To elucidate the regulatory mechanisms underlying the expression of DFRs, we investigated the transcriptional control of each member of the DFR multigene family. Ectopic expression of a combination of the transcription factors MYB, bHLH, and WDR showed that only the DFR2 promoter was activated, indicating that each member of the DFR gene family is regulated independently.
在模式豆科植物百脉根的基因组中，二氢黄酮醇 4-还原酶（DFR）是花青素和原花青素（PA）途径的第一个关键酶，它被编码为串联排列的五个基因家族。表达分析表明，DFR 在器官特异性和应激响应方面存在差异。为了阐明 DFR 表达的调控机制，我们研究了 DFR 多基因家族的每个成员的转录调控。MYB、bHLH 和 WDR 转录因子的异位表达表明，只有 DFR2 启动子被激活，这表明 DFR 基因家族的每个成员都是独立调控的。
