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L.中基因家族的鉴定与表达分析

Identification and Expression Analysis of Gene Family in L.

作者信息

Qian Xingzhi, Zheng Wenyin, Hu Jian, Ma Jinxu, Sun Mengyuan, Li Yong, Liu Nian, Chen Tianhua, Wang Meiqi, Wang Ling, Hou Xinzhe, Cai Qingao, Ye Zhaoshun, Zhang Fugui, Zhu Zonghe

机构信息

College of Agronomy, Anhui Agricultural University, Hefei 230036, China.

出版信息

Plants (Basel). 2023 Jul 7;12(13):2583. doi: 10.3390/plants12132583.

DOI:10.3390/plants12132583
PMID:37447144
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10346495/
Abstract

Dihydroflavonol 4-reductase () is a key enzyme in the flavonoid biosynthetic pathway and is essential for the formation of plants' color. In this study, 26 genes were identified using 6 genes as reference. The physicochemical properties, subcellular localization, and conserved structure of proteins were analyzed; the evolutionary relationship, collinearity analysis, and expression characteristics of genes were studied; and the correlation between the expression level of genes and anthocyanin content in rape petals were analyzed. The results showed that the 26 were located in chloroplasts, cytoplasm, nuclei, and mitochondria, distributed on 17 chromosomes, and divided into 4 groups; members of the same group have a similar function, which may be related to the environmental response elements and plant hormone response elements. Intraspecific collinearity analysis showed 51 pairs of collinear genes, and interspecific collinearity analysis showed 30 pairs of collinear genes. Analysis of the expression levels of and anthocyanin content in different color rape petals showed that and might play an important role in the synthesis of anthocyanins in rape petals. This provides theoretical guidance for further analysis of the anthocyanin anabolism mechanism involved in the gene in .

摘要

二氢黄酮醇4-还原酶（）是类黄酮生物合成途径中的关键酶，对植物颜色的形成至关重要。在本研究中，以6个基因作为参考鉴定出26个基因。分析了这些蛋白质的理化性质、亚细胞定位和保守结构；研究了这些基因的进化关系、共线性分析和表达特征；并分析了这些基因的表达水平与油菜花瓣中花青素含量之间的相关性。结果表明，这26个基因位于叶绿体、细胞质、细胞核和线粒体中，分布在17条染色体上，分为4组；同一组的成员具有相似的功能，这可能与环境响应元件和植物激素响应元件有关。种内共线性分析显示有51对共线基因，种间共线性分析显示有30对共线基因。对不同颜色油菜花瓣中这些基因的表达水平和花青素含量的分析表明，这些基因可能在油菜花瓣花青素的合成中起重要作用。这为进一步分析油菜中该基因参与的花青素合成代谢机制提供了理论指导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/e44d4f998dde/plants-12-02583-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/59559fbdaf91/plants-12-02583-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/04610dc0b8b9/plants-12-02583-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/122c9f0d1e37/plants-12-02583-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/5a4107b19141/plants-12-02583-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/bdfcd34483d0/plants-12-02583-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/8a0443fac7c4/plants-12-02583-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/82c922ea1545/plants-12-02583-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/e44d4f998dde/plants-12-02583-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/59559fbdaf91/plants-12-02583-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/04610dc0b8b9/plants-12-02583-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/122c9f0d1e37/plants-12-02583-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/5a4107b19141/plants-12-02583-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/bdfcd34483d0/plants-12-02583-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/8a0443fac7c4/plants-12-02583-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/82c922ea1545/plants-12-02583-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8375/10346495/e44d4f998dde/plants-12-02583-g008.jpg

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