雌激素受体 α 和 β 在胃癌中的预后作用。

Prognostic role of estrogen receptor alpha and estrogen receptor beta in gastric cancer.

机构信息

Department of Surgical Oncology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

出版信息

Ann Surg Oncol. 2010 Sep;17(9):2503-9. doi: 10.1245/s10434-010-1031-2. Epub 2010 Mar 26.

DOI:10.1245/s10434-010-1031-2

PMID:20339947

Abstract

BACKGROUND

The presence of estrogen receptor alpha (ERalpha) and estrogen receptor beta (ERbeta) have been reported in cell and tissue level in gastric cancer, but its impact on patients' survival remains unclear. This study was designed to investigate the expression level of ERalpha and ERbeta and to assess clinical significance of ERalpha and ERbeta expression in gastric cancer.

METHODS

The expression level of ERalpha and ERbeta were assessed by reverse-transcriptase polymerase chain reaction (RT-PCR) in 35 surgically resected gastric cancer and corresponding normal tissues and by immunohistochemical staining in 211 surgically resected gastric cancer and match normal tissues.

RESULTS

The expression level between ERalpha mRNA expression in gastric cancer tissues and match normal tissues had no statistically significant difference. The ERbeta mRNA level in normal tissues was significantly higher than that observed in gastric cancer tissues (P = 0.001). Neither ERalpha nor ERbeta mRNA expression levels had significant correlation with clinicopathologic parameters. Forty-eight of 211 (22.7%) gastric cancer tissues showed positive expression of ERalpha and ERbeta detected in gastric cancer. ERalpha-positive expression correlated with poorer overall survival (P = 0.014), as did the absence of ERbeta expression in patients with gastric cancer (P = 0.001). In multivariate analysis, the positive expression of ERalpha and the absence of ERbeta were significant independent prognostic factors for overall survival (hazard ratio 2.159, P = 0.013, and hazard ratio 2.016, P = 0.025 respectively).

CONCLUSIONS

Our results indicated that ERalpha and ERbeta were expressed in both gastric cancer and corresponding normal tissues. ERalpha expression and the absence of ERbeta expression are associated with poor survival.

摘要

背景

雌激素受体α（ERα）和雌激素受体β（ERβ）已在胃癌的细胞和组织水平上被报道，但它们对患者生存的影响尚不清楚。本研究旨在调查 ERα和 ERβ的表达水平，并评估 ERα和 ERβ在胃癌中的表达的临床意义。

方法

通过逆转录聚合酶链反应（RT-PCR）在 35 例手术切除的胃癌和相应的正常组织中评估 ERα和 ERβ的表达水平，并通过免疫组织化学染色在 211 例手术切除的胃癌和匹配的正常组织中评估 ERα和 ERβ的表达水平。

结果

胃癌组织和匹配正常组织中 ERα mRNA 表达水平之间无统计学显著差异。正常组织中的 ERβ mRNA 水平明显高于胃癌组织（P = 0.001）。ERα 或 ERβ mRNA 表达水平与临床病理参数均无显著相关性。在 211 例胃癌组织中，有 48 例（22.7%）检测到 ERα 和 ERβ 的阳性表达。ERα 阳性表达与总体生存率较差相关（P = 0.014），胃癌患者中 ERβ 表达缺失也是如此（P = 0.001）。在多变量分析中，ERα 的阳性表达和 ERβ 的缺失是总体生存率的显著独立预后因素（危险比 2.159，P = 0.013 和危险比 2.016，P = 0.025）。