Department of Orthopaedics Unit 3, Christian Medical College, Vellore, Vellore, Tamil Nadu, India.
Int Orthop. 2011 Jan;35(1):143-8. doi: 10.1007/s00264-010-0994-8. Epub 2010 Mar 26.
This study aimed to determine the efficacy of PEMF (pulsed electromagnetic field) treatment in experimental osteochondral defect healing in a rabbit model. The study was conducted on 12 New Zealand white rabbits. Six rabbits formed the study group and six rabbits the control group. The right knee joints of all 12 animals were exposed and a 3.5-mm diameter osteochondral defect was created in the trochlear groove. The defect was filled with calcium phosphate scaffold. Six animals from the study group were given PEMF of one hour duration once a day for six weeks with set parameters for frequency of 1 Hz, voltage 20 V, sine wave and current ±30 mA. At six weeks the animals were sacrificed and histological evaluation was done using H&E, Safranin O, Maissons trichrome staining and immunohistochemistry for type 2 collagen. The quality of the repair tissue was graded and compared between groups with the Wakitani histological grading scale and a statistical analysis was done. The total histological score was significantly better in the study group (p = 0.002) with regeneration similar to adjacent normal hyaline cartilage. Immunohistochemistry for collagen type II was positive in the study group. PEMF stimulation of osteochondral defects with calcium phosphate scaffold is effective in hyaline cartilage formation. PEMF is a non-invasive and cost effective adjuvant treatment with salvage procedures such as abrasion chondroplasty and subchondral drilling.
本研究旨在确定脉冲电磁场(PEMF)治疗在兔模型实验性骨软骨缺损愈合中的疗效。该研究共纳入 12 只新西兰白兔。其中 6 只形成研究组,6 只形成对照组。所有 12 只动物的右膝关节暴露,在滑车沟处造成 3.5mm 直径的骨软骨缺损。缺损处填充磷酸钙支架。研究组中 6 只动物每天接受 1 小时的 PEMF 治疗,持续 6 周,频率为 1Hz,电压 20V,正弦波,电流±30mA。6 周后处死动物,采用 H&E、番红 O、马森三色染色和 II 型胶原免疫组化进行组织学评估。使用 Wakitani 组织学分级标准对修复组织的质量进行分级,并对两组进行比较,同时进行统计学分析。研究组的总组织学评分明显更好(p=0.002),再生组织类似于相邻正常透明软骨。研究组的 II 型胶原免疫组化呈阳性。PEMF 刺激磷酸钙支架的骨软骨缺损在透明软骨形成中是有效的。PEMF 是一种非侵入性、具有成本效益的辅助治疗方法,可联合采用磨蚀软骨成形术和软骨下钻孔等挽救性手术。
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