Anbarasan Selvam, Baraneedharan Ulaganathan, Paul Solomon Fd, Kaur Harpreet, Rangaswami Subramoniam, Bhaskar Emmanuel
Department of Human Genetics, Sri Ramachandra University, Porur, Chennai, Tamil Nadu, India.
Department of Biomedical Sciences, Sri Ramachandra University, Porur, Chennai, Tamil Nadu, India.
Indian J Orthop. 2016 Jan-Feb;50(1):87-93. doi: 10.4103/0019-5413.173522.
Pulsed electromagnetic field (PEMF) is used to treat bone and joint disorders for over 30 years. Recent studies demonstrate a significant effect of PEMF on bone and cartilage proliferation, differentiation, synthesis of extracellular matrix (ECM) and production of growth factors. The aim of this study is to assess if PEMF of low frequency, ultralow field strength and short time exposure have beneficial effects on in-vitro cultured human chondrocytes.
Primary human chondrocytes cultures were established using articular cartilage obtained from knee joint during joint replacement surgery. Post characterization, the cells were exposed to PEMF at frequencies ranging from 0.1 to 10 Hz and field intensities ranging from 0.65 to 1.95 μT for 60 min/day for 3 consecutive days to analyze the viability, ECM component synthesis, proliferation and morphology related changes post exposure. Association between exposure doses and cellular effects were analyzed with paired't' test.
In-vitro PEMF exposure of 0.1 Hz frequency, 1.95 μT and duration of 60 min/day for 3 consecutive days produced the most favorable response on chondrocytes viability (P < 0.001), ECM component production (P < 0.001) and multiplication. Exposure of identical chondrocyte cultures to PEMFs of 0.65 μT field intensity at 1 Hz frequency resulted in less significant response. Exposure to 1.3 μT PEMFs at 10 Hz frequency does not show any significant effects in different analytical parameters.
Short duration PEMF exposure may represent a new therapy for patients with Osteoarthritis (OA).
脉冲电磁场（PEMF）用于治疗骨和关节疾病已有30多年。最近的研究表明，PEMF对骨和软骨的增殖、分化、细胞外基质（ECM）合成及生长因子产生具有显著影响。本研究的目的是评估低频、超低场强和短时间暴露的PEMF对体外培养的人软骨细胞是否有有益作用。
使用关节置换手术中从膝关节获取的关节软骨建立原代人软骨细胞培养物。在进行特性鉴定后，将细胞暴露于频率范围为0.1至10Hz、场强范围为0.65至1.95μT的PEMF下，每天暴露60分钟，连续3天，以分析暴露后细胞活力、ECM成分合成、增殖和形态相关变化。采用配对t检验分析暴露剂量与细胞效应之间的关联。
连续3天每天暴露于频率为0.1Hz、场强为1.95μT、持续时间为60分钟的体外PEMF，对软骨细胞活力（P<0.001）、ECM成分产生（P<0.001）和增殖产生了最有利的反应。将相同的软骨细胞培养物暴露于频率为1Hz、场强为0.65μT的PEMF下，反应则不太明显。暴露于频率为10Hz、场强为1.3μT的PEMF在不同分析参数中未显示任何显著影响。
短时间暴露于PEMF可能为骨关节炎（OA）患者提供一种新的治疗方法。
