急性脑梗死患者循环凋亡因子。

Circulating apoptotic factors in patients with acute cerebral infarction.

机构信息

Department of Bioresources, DaYeh University, Changhua, Taiwan.

出版信息

Clin Biochem. 2010 Jun;43(9):761-3. doi: 10.1016/j.clinbiochem.2010.03.002. Epub 2010 Mar 27.

DOI:10.1016/j.clinbiochem.2010.03.002

PMID:20347759

Abstract

OBJECTIVE

Studies on animal models have shown that apoptosis plays a role in acute ischemic stroke, but the clinical significance of apoptosis in human stroke has not been well established.

DESIGN AND METHODS

Acute ischemic stroke patients who had been examined by magnetic resonance imaging (MRI) were recruited, and their blood samples were collected after informed consent obtained. Sixteen samples were taken from 7 patients and 4 control subjects. DNA was extracted from the plasma and analyzed. The plasma level of tumor necrosis factor-alpha (TNF-alpha) was analyzed by western blot, and relative caspase 3/7 activity was assayed.

RESULTS

Soluble and membrane-associated TNF-alpha were found to be higher in the plasma from ischemic stroke patients than in that from the controls. Further, this result was more significant in patients with grey matter lesions than white matter lesions.

CONCLUSIONS

Apoptotic factors are detected in the plasma of patients with acute ischemic stroke and might be related to infarct area.

摘要

目的

动物模型研究表明，细胞凋亡在急性缺血性脑卒中中有一定作用，但细胞凋亡在人类脑卒中中的临床意义尚未得到充分证实。

设计与方法

选取经磁共振成像（MRI）检查的急性缺血性脑卒中患者，并在获得知情同意后采集其血样。从 7 名患者和 4 名对照者中采集了 16 份样本。从血浆中提取 DNA 并进行分析。采用 Western blot 法分析肿瘤坏死因子-α（TNF-α）的血浆水平，并测定相对半胱氨酸天冬氨酸蛋白酶 3/7 活性。