Bezlepkin V G, Ostrovskaia L B, Bezlepkina T A, Giaziev A I
Radiobiologiia. 1991 Mar-Apr;31(2):188-94.
In experiments with NIH3T3 mouse fibroblast the authors showed a dose-dependent (gamma- or UV-radiation) increase in the immortalized fibroblast transformation by purified DNA preparations from Ehrlich ascites tumour cells. The transformant yield was the highest when the transfection started within the first few minutes after irradiation, when radiation lesions occurred in the genome and the system of enzymic DNA repair was activated. The proteolytic activity inhibition by treating the exposed cells with phenylmethanesultonyl fluorine reduced the radiation-induced transformation.
在对NIH3T3小鼠成纤维细胞进行的实验中，作者发现，用艾氏腹水瘤细胞的纯化DNA制剂处理后，永生化成纤维细胞的转化呈剂量依赖性（γ射线或紫外线辐射）增加。当在辐射后的最初几分钟内开始转染时，转化体产量最高，此时基因组中出现辐射损伤，并且酶促DNA修复系统被激活。用苯甲基磺酰氟处理受照射细胞抑制蛋白水解活性，可降低辐射诱导的转化。
