用于检测和测定牛体内杀锥虫药物氯异喹胺的酶联免疫吸附测定法的开发。

Development of an enzyme-linked immunosorbent assay for the detection and measurement of the trypanocidal drug isometamidium chloride in cattle.

作者信息

Whitelaw D D, Gault E A, Holmes P H, Sutherland I A, Rowell F J, Phillips A, Urquhart G M

机构信息

University of Glasgow, Veterinary School.

出版信息

Res Vet Sci. 1991 Mar;50(2):185-9. doi: 10.1016/0034-5288(91)90103-u.

DOI:10.1016/0034-5288(91)90103-u

PMID:2034897

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed to measure accurately levels of the trypanocidal drug isometamidium in the serum of treated cattle. The assay requires only 5 microliters of test serum, is sensitive to a level of 0.5 pg ml-1 and is highly specific. Cross reactivity does not occur with the two other widely used trypanocidal drugs diminazene aceturate and homidium bromide. Serum drug levels are detectable for up to six months in cattle after a single dose of 1 mg kg-1 intramuscularly, the maximum period under field conditions for which effective prophylaxis can be maintained against tsetse challenge. Application of the assay will aid the rationalisation of treatment campaigns and assist in assessing the occurrence of drug-resistant trypanosome populations.

摘要

开发了一种酶联免疫吸附测定法（ELISA），用于准确测量经治疗牛血清中杀锥虫药物异美汀的水平。该测定法仅需5微升测试血清，对0.5 pg ml-1的水平敏感且具有高度特异性。与另外两种广泛使用的杀锥虫药物乙酰氨基阿的平和溴化百浪多息不会发生交叉反应。单次肌内注射1 mg kg-1后，牛血清中的药物水平在长达六个月内均可检测到，这是在野外条件下能够维持对采采蝇攻击有效预防的最长时间。该测定法的应用将有助于治疗活动的合理化，并有助于评估耐药锥虫群体的出现情况。