Peregrine A S, Eisler M C, Katende J, Flynn J N, Gault E A, Kinabo L D, Holmes P H
International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya.
Hybridoma. 1994 Aug;13(4):289-94. doi: 10.1089/hyb.1994.13.289.
Mice were immunized with either an isometamidium-human serum albumin (HSA) conjugate or an isometamidium-porcine thyroglobulin conjugate (PTG). Thereafter, monoclonal antibodies (MAbs) IL-A 1001, IL-A 1002, IL-A 1003, 5F7.B7, and 5F7.C9 were generated and selected on the basis that they recognized conjugated and unconjugated isometamidium, but lacked cross-reactivity with the carrier molecules. All five MAbs were of the IgG1 isotype. Each of the five MAbs was assessed in a competitive ELISA for isometamidium; in each case, the minimum level of detection was approximately 10 ng/ml. Each MAb exhibited approximately 0.1% cross-reactivity with the anti-trypanosomal compound diminazene. However, based on their cross-reactivity with the anti-trypanosomal compound homidium, the MAbs could be divided into two groups; IL-A 1001, IL-A 1002, and IL-A 1003, produced using an isometamidium-HSA conjugate as an immunogen, exhibited low levels of cross-reactivity (approximately 0.1%). In contrast, 5F7.B7 and 5F7.C9, produced using an isometamidium-PTG conjugate as an immunogen, exhibited high levels of cross-reactivity.
用异硫氰酸甲脒-人血清白蛋白（HSA）偶联物或异硫氰酸甲脒-猪甲状腺球蛋白偶联物（PTG）对小鼠进行免疫。此后，产生了单克隆抗体（MAb）IL-A 1001、IL-A 1002、IL-A 1003、5F7.B7和5F7.C9，并基于它们能识别偶联和未偶联的异硫氰酸甲脒，但与载体分子缺乏交叉反应性而进行选择。所有五种单克隆抗体均为IgG1同种型。对这五种单克隆抗体中的每一种进行了异硫氰酸甲脒竞争性ELISA检测；在每种情况下，最低检测水平约为10 ng/ml。每种单克隆抗体与抗锥虫化合物二脒那嗪表现出约0.1%的交叉反应性。然而，基于它们与抗锥虫化合物甲硝哒唑的交叉反应性，这些单克隆抗体可分为两组；使用异硫氰酸甲脒-HSA偶联物作为免疫原产生的IL-A 1001、IL-A 1002和IL-A 1003表现出低水平的交叉反应性（约0.1%）。相比之下，使用异硫氰酸甲脒-PTG偶联物作为免疫原产生的5F7.B7和5F7.C9表现出高水平的交叉反应性。
