ARC Centre of Excellence for Free Radical Chemistry, School of Physical and Chemical Sciences, Queensland University of Technology, Brisbane, QLD 4001, Australia.
Free Radic Biol Med. 2010 Jul 1;49(1):67-76. doi: 10.1016/j.freeradbiomed.2010.03.019. Epub 2010 Mar 27.
Changes to the redox status of biological systems have been implicated in the pathogenesis of a wide variety of disorders. Sensitive quantification of these changes has been developed using a novel fluorescent probe containing a redox-sensitive nitroxide moiety. As well as being able to selectively detect the superoxide radical in vitro, this method can measure overall changes to the cellular redox environment using flow cytometry on the basis of nitroxide reduction. The reversible nature of the probe's detection mechanism offers the unique advantage of being able to monitor redox changes in both oxidizing and reducing directions in real time.
生物系统氧化还原状态的变化与多种疾病的发病机制有关。使用含有氧化还原敏感硝氧自由基部分的新型荧光探针，已经开发出对这些变化进行敏感定量的方法。该方法不仅能够在体外选择性地检测超氧自由基，还可以基于硝氧自由基的还原，使用流式细胞术测量细胞氧化还原环境的整体变化。探针检测机制的可逆性质提供了一个独特的优势，即能够实时监测氧化和还原两个方向的氧化还原变化。
