Matsuoka Yuta
Physical Chemistry for Life Science Laboratory, Faculty of Pharmaceutical Sciences, Kyushu University.
Yakugaku Zasshi. 2021;141(12):1297-1304. doi: 10.1248/yakushi.21-00149.
Disruption of the redox balance in vivo is closely involved in the development of various diseases associated with oxidative stress. Therefore, methods for the in vivo analysis of antioxidants and free radicals are essential to elucidate the pathogenic mechanisms of such diseases. Although profluorescent nitroxide probes can be used to evaluate redox molecules with high sensitivity, these probes have low selectivity. Recently, we developed two profluorescent nitroxide probes, 15-((9-(ethylimino)-10-methyl-9Hbenzo[a]phenoxazin-5-yl)amino)-3,11-dioxa-7-azadispiro-hexadecan-7-yloxyl (Nile-DiPy) and 2,2,6-trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperidine-1-oxyl (NBD-Pen), which had high sensitivity and selectivity toward ascorbic acid and lipid-derived radicals, respectively. These probes can react sensitively and selectively to each target molecule and can be used in animal experiments. In this paper, we review the design strategies and application of these profluorescent nitroxide probes.
体内氧化还原平衡的破坏与各种与氧化应激相关的疾病的发展密切相关。因此，用于体内抗氧化剂和自由基分析的方法对于阐明此类疾病的致病机制至关重要。尽管前荧光氮氧化物探针可用于高灵敏度地评估氧化还原分子，但这些探针的选择性较低。最近，我们开发了两种前荧光氮氧化物探针，15-((9-(乙基亚氨基)-10-甲基-9H-苯并[a]吩恶嗪-5-基)氨基)-3,11-二氧杂-7-氮杂双螺[十六烷]-7-基氧基(尼罗-二吡啶)和2,2,6-三甲基-4-(4-硝基苯并[1,2,5]恶二唑-7-基氨基)-6-戊基哌啶-1-氧基(NBD-戊基)，它们分别对抗坏血酸和脂质衍生的自由基具有高灵敏度和选择性。这些探针可以对每个目标分子进行灵敏且选择性的反应，并可用于动物实验。在本文中，我们综述了这些前荧光氮氧化物探针的设计策略和应用。
