Faculty of Health Sciences, Department of Health, Course of Acupuncture and Moxibustion, Tsukuba University of Technology, National University Corporation, Kasuga Ibaraki, Japan.
Acupunct Med. 2010 Mar;28(1):21-4. doi: 10.1136/aim.2008.000257.
To determine if there is any difference in pupillary response among different acupuncture stimulation sites.
The subjects were 14 healthy males who had no known eye diseases or abnormality in their pupils. They received five different interventions: no acupuncture stimulation (hereinafter 'no-stimulation') and acupuncture stimulation at four sites (TE5, ST7, CV12 and ST36). The Latin square design was used to allocate stimulation order. For all acupuncture stimulation interventions, a disposable acupuncture needle was inserted superficially at the acupuncture point. Gentle repetitive tapping stimulation was applied manually during the subject's exhalation phase of respiration, for 90 s. The pupil diameter was continuously measured for 2 min before stimulation, during stimulation and for 2 min after stimulation. Statistical analysis was conducted on serial changes in pupil diameter during acupuncture stimulation on each respective site and during non-stimulation session by analysis of variance and Fisher (least significant difference) multiple comparison, with linear analysis using a mixed model.
Pupil diameter reduction occurred at 30 s after stimulation on ST7 (p=0.008) and 60 s after stimulation (p=0.014) compared with pre-stimulation. The decrease of pupillary diameter occurred 60 s after stimulation on TE5 (p=0.028) compared with pre-stimulation. On ST36, CV12 and during the non-stimulation intervention, no significant change in the pupil diameter was observed.
Pupillary reaction varies depending on the different stimulation sites.
确定不同针刺刺激部位的瞳孔反应是否存在差异。
本研究纳入了 14 名健康男性，他们均无已知的眼部疾病或瞳孔异常。他们接受了 5 种不同的干预措施：无针刺刺激（以下简称“无刺激”）和 4 个穴位的针刺刺激（TE5、ST7、CV12 和 ST36）。采用拉丁方设计来分配刺激顺序。对于所有针刺刺激干预，一次性针灸针在穴位处浅层插入。在受试者呼气阶段，手动轻柔地重复轻敲刺激，持续 90s。在刺激前、刺激期间和刺激后 2 分钟连续测量瞳孔直径。采用方差分析和 Fisher（最小显著差异）多重比较，对每个穴位和非刺激期间的针刺刺激过程中瞳孔直径的连续变化进行统计分析，线性分析采用混合模型。
与刺激前相比，ST7 在刺激后 30s（p=0.008）和 60s（p=0.014）时瞳孔直径减小，TE5 在刺激后 60s（p=0.028）时瞳孔直径减小。在 ST36、CV12 和非刺激干预期间，瞳孔直径没有明显变化。
瞳孔反应因刺激部位的不同而有所差异。
