Chunyan Chi, Bo Shi, Ping Liang, Jingmei Li, Ito Yoichiro
Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
J Liq Chromatogr Relat Technol. 2009 Jan 1;32(1):136-143. doi: 10.1080/10826070802548747.
High-speed countercurrent chromatography was successfully applied for the first time for the separation of psoralen and bergapten from Ficus carica L leaves. The crudeextract obtained by light petroleum (b.p.: 60°C-90°C) from the dried leaves of Ficus carica L. was separated with a two-phase solvent system of n-Hexane-ethyl acetate-methanol-water (1:1:1:1, v/v). Each peak fraction was analyzed by high-performance liquid chromatography. The method yielded 4.4 mg of psoralen at 99.1% purity and 2.1 mg of bergapten at 98.2% purity from 400 mg of the crude extract in a single run. The two compounds were identified by (1)H-NMR, (13)C-NMR and MS.
高速逆流色谱法首次成功应用于从无花果叶中分离补骨脂素和佛手柑内酯。用轻质石油醚(沸点:60℃-90℃)从无花果干燥叶中获得的粗提物,采用正己烷-乙酸乙酯-甲醇-水(1:1:1:1,v/v)两相溶剂体系进行分离。每个峰馏分通过高效液相色谱法进行分析。该方法单次运行从400mg粗提物中得到纯度为99.1%的4.4mg补骨脂素和纯度为98.2%的2.1mg佛手柑内酯。这两种化合物通过(1)H-NMR、(13)C-NMR和MS进行鉴定。