SEPT7 的表达模式与精子形态相关。
The expression pattern of SEPT7 correlates with sperm morphology.
机构信息
Division of Obstetrics and Gynecology, National Cheng Kung University College of Medicine and Hospital, Dou-Liou Branch, Yunlin, Taiwan.
出版信息
J Assist Reprod Genet. 2010 Jun;27(6):299-307. doi: 10.1007/s10815-010-9409-4. Epub 2010 Mar 30.
PURPOSE
To investigate the expression pattern of the SEPT7 protein during spermatogenesis and its potential role in sperm function.
METHODS
We first investigated the expression pattern of SEPT7 during different steps of mouse spermiogenesis using an immunofluorescence assay (IFA). IFA was also applied to study the expression pattern of SEPT7 in human ejaculated spermatozoa. Nine fertile men with normal semen parameters were used as the control group, and 21 infertile men with asthenozoospermia were recruited as the patient group. We assessed the frequency of the SEPT7 signal in the various morphological subgroups.
RESULTS
In humans, the frequency of a defective SEPT7 signal was significantly increased in men with asthenozoospermia. The absence of a SEPT7 signal was more prevalent in sperm containing morphological defects of various types.
CONCLUSIONS
The expression pattern of SEPT7 suggested that this protein may be involved in the regulation of subcellular-compartment formation during spermiogenesis in the mouse. The absence of a SEPT7 signal correlated with multiple sperm defects.
目的
研究 SEPT7 蛋白在精子发生过程中的表达模式及其在精子功能中的潜在作用。
方法
我们首先使用免疫荧光法（IFA）研究 SEPT7 在小鼠精子发生的不同阶段的表达模式。IFA 还用于研究 SEPT7 在人射出精子中的表达模式。我们使用 9 名具有正常精液参数的生育男性作为对照组，招募 21 名具有弱精症的不育男性作为患者组。我们评估了 SEPT7 信号在不同形态亚群中的出现频率。
结果
在人类中，弱精症患者的 SEPT7 信号缺陷频率显著增加。在含有各种形态缺陷的精子中，SEPT7 信号缺失更为普遍。
结论
SEPT7 的表达模式表明，该蛋白可能参与了小鼠精子发生过程中细胞亚区形成的调节。SEPT7 信号缺失与多种精子缺陷相关。
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