Hosseinifar Hani, Shafipour Maryam, Modarresi Tahereh, Azad Mahnaz, Sadighi Gilani Mohammad Ali, Shahhosseini Maryam, Sabbaghian Marjan
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
J Assist Reprod Genet. 2014 Dec;31(12):1681-5. doi: 10.1007/s10815-014-0353-6. Epub 2014 Oct 10.
To find a relationship between absence of annulus and asthenozoospermia in Iranian men.
In the present study, semen samples from 100 asthenozoospermic and 20 normozospermic patients were analyzed for sperm concentration and motility. Spermatozoa were immunostained for the two septin subunits Sept4 and Sept7. The absence of the annulus structure was confirmed by transmission electron microscopy and western blot analysis for septin 4. DNA sequencing for all coding exons of SEPT12 was performed for a patient using peripheral blood sample.
Specific antibodies for SEPT4 and SEPT7 consistently labeled the annuli in spermatozoa from all of the 20 normozospermic men, while in one of 100 patients with asthenozoospermia, 75% of sperms lacking septin 4 or septin 7 proteins at the annulus. It was shown that the structural defect in annulus formation is not caused by point mutation of SEPT12 gene.
In conclusion, the results of this study demonstrated that the frequency of the absence of annulus in asthenozoospermic sample of Iranian population has a low frequency and could not be assume as a diagnostic marker for classifying asthenozoospermic patients.
探寻伊朗男性中环缺失与弱精子症之间的关系。
在本研究中，对100例弱精子症患者和20例正常精子症患者的精液样本进行精子浓度和活力分析。对精子进行Sept4和Sept7这两种septin亚基的免疫染色。通过透射电子显微镜和septin 4的蛋白质印迹分析确认环结构的缺失。使用外周血样本对一名患者的SEPT12所有编码外显子进行DNA测序。
SEPT4和SEPT7的特异性抗体始终标记20例正常精子症男性所有精子中的环，而在100例弱精子症患者中的1例中，75%的精子在环处缺乏septin 4或septin 7蛋白。结果表明，环形成的结构缺陷不是由SEPT12基因的点突变引起的。
总之，本研究结果表明，伊朗人群弱精子症样本中环缺失的频率较低，不能将其作为弱精子症患者分类的诊断标志物。
