泰国犬细小病毒分离株VP2基因的序列分析
Sequence analysis of VP2 gene of canine parvovirus isolates in Thailand.
作者信息
Phromnoi Siriwadee, Sirinarumitr Kaitkanoke, Sirinarumitr Theerapol
机构信息
Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, 73140, Thailand.
出版信息
Virus Genes. 2010 Aug;41(1):23-9. doi: 10.1007/s11262-010-0475-6. Epub 2010 Mar 30.
Canine parvovirus (CPV) causes a very severe enteric disease especially in puppies. Twenty-six isolates of CPV were obtained from dogs at the Animal Hospital, Kasetsart University, Thailand. Whole VP2 gene of 26 isolates was amplified using polymerase chain reaction (PCR) and its sequences were analyzed. Nineteen out of 26 isolates were characterized as CPV type 2a variants and the rest of the isolates were characterized as CPV type 2b. These results indicated that both types are currently prevalent field CPV circulating in Thailand and type 2a is the predominant genotype. Neither CPV type 2 nor type 2c was observed in this study.
犬细小病毒（CPV）会引发一种非常严重的肠道疾病，尤其是在幼犬中。从泰国农业大学动物医院的犬只身上获取了26株犬细小病毒分离株。使用聚合酶链反应（PCR）扩增了这26株分离株的整个VP2基因，并对其序列进行了分析。26株分离株中有19株被鉴定为2a型犬细小病毒变体，其余分离株被鉴定为2b型。这些结果表明，这两种类型目前都是在泰国流行的犬细小病毒野外毒株，且2a型是主要的基因型。本研究中未观察到2型或2c型犬细小病毒。
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