Prevalence and molecular characterization of canine parvovirus.

BACKGROUND AND AIM

Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to study molecular epidemiology of CPV by sequence analysis of gene to determine the prevailing antigenic type(s) in the northern regions of India.

MATERIALS AND METHODS

A total of 118 rectal swabs collected from dogs exhibiting clinical signs of CPV infection were processed for the isolation of DNA and subjected to polymerase chain reaction (PCR) and nested PCR (NPCR). A total of 13 NPCR products selected randomly were subjected to sequence analysis of gene.

RESULTS

The percent positivity of CPV was found 28% and 70% by PCR and NPCR, respectively. Dogs with vaccination history against CPV too were found positive with a percent positivity of 24.10%. Gene sequencing and phylogenetic analysis of gene from these isolates revealed that most samples formed a clade with CPV-2a isolates.

CONCLUSION

Sequence analysis and phylogenetic analysis of gene in the studied regions of northern India revealed that CPV-2a was the most prevalent antigenic type.