Singh Parikshit, Kaur Gurpreet, Chandra Mudit, Dwivedi P N
Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Vet World. 2021 Mar;14(3):603-606. doi: 10.14202/vetworld.2021.603-606. Epub 2021 Mar 9.
Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to study molecular epidemiology of CPV by sequence analysis of gene to determine the prevailing antigenic type(s) in the northern regions of India.
A total of 118 rectal swabs collected from dogs exhibiting clinical signs of CPV infection were processed for the isolation of DNA and subjected to polymerase chain reaction (PCR) and nested PCR (NPCR). A total of 13 NPCR products selected randomly were subjected to sequence analysis of gene.
The percent positivity of CPV was found 28% and 70% by PCR and NPCR, respectively. Dogs with vaccination history against CPV too were found positive with a percent positivity of 24.10%. Gene sequencing and phylogenetic analysis of gene from these isolates revealed that most samples formed a clade with CPV-2a isolates.
Sequence analysis and phylogenetic analysis of gene in the studied regions of northern India revealed that CPV-2a was the most prevalent antigenic type.
犬细小病毒（CPV）属于细小病毒科，可引起犬出血性胃肠炎，并导致幼犬高死亡率。该病毒有三种结构蛋白（VP1、VP2和VP3）和两种非结构蛋白（NS1和NS2），其中VP2具有高度免疫原性。本研究旨在通过对基因进行序列分析来研究CPV的分子流行病学，以确定印度北部地区流行的抗原类型。
从表现出CPV感染临床症状的犬只中收集了118份直肠拭子，用于DNA分离，并进行聚合酶链反应（PCR）和巢式PCR（NPCR）。随机选择13个NPCR产物进行基因序列分析。
通过PCR和NPCR检测，CPV的阳性率分别为28%和70%。有CPV疫苗接种史的犬只也呈阳性，阳性率为24.10%。对这些分离株的基因进行测序和系统发育分析表明，大多数样本与CPV-2a分离株形成一个分支。
对印度北部研究区域的基因进行序列分析和系统发育分析表明，CPV-2a是最流行的抗原类型。
