Jeoung Seok-Young, Ahn So-Jeo, Kim Doo
School of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon, Kangwon, Republic of Korea.
J Vet Med Sci. 2008 Jul;70(7):719-22. doi: 10.1292/jvms.70.719.
The purpose of this study was to genetically characterize CPV isolates from Korea. The VP2 gene of 31 isolates was characterized by DNA sequencing and their phylogeny. Among the 31 field CPV isolates, 28 isolates were classified as type 2a and other 3 isolates as type 2b. The isolates in 2a-I, II and III subclusters have unique mutations. The isolates in 2a-IV and V subclusters had similar amino acid sequences to type 2a isolates from other parts of the world. The isolates in type 2b had similar amino acid sequences to type 2b isolates from Asia, Italy, and U.S.A. The molecular analysis of VP2 gene of CPV provided the useful information for the identification of CPV types and the understanding of their genetic relationship.
本研究的目的是对来自韩国的犬细小病毒（CPV）分离株进行基因特征分析。通过DNA测序及其系统发育分析对31株分离株的VP2基因进行了特征分析。在31株CPV野外分离株中，28株被归类为2a型，另外3株为2b型。2a-I、II和III亚群中的分离株具有独特的突变。2a-IV和V亚群中的分离株与来自世界其他地区的2a型分离株具有相似的氨基酸序列。2b型中的分离株与来自亚洲、意大利和美国的2b型分离株具有相似的氨基酸序列。CPV VP2基因的分子分析为CPV类型的鉴定及其遗传关系的理解提供了有用信息。
