表面增强激光解吸电离飞行时间质谱蛋白质芯片技术在阴虚内热证系统性红斑狼疮诊断中的应用研究

[Study on application of SELDI protein chip technique in diagnosis of systemic lupus erythematosus of yin deficiency caused internal heat syndrome].

作者信息

Lai Mei-sheng, Fan Rui-qiang

机构信息

Surgery of Traditional Chinese Medicine Department, College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Jan;30(1):26-9.

PMID:20353027

Abstract

OBJECTIVE

To investigate the application of surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI) protein chip in diagnosis and Chinese medicie syndrome type researching of systemic lupus erythematosus (SLE).

METHODS

Eighteen female SLE patients of mild/moderate degree with yin-deficiency caused internal heat syndrome (YDHS) were enrolled in the treatment group, and 15 women healthy volunteers was set up as the control group. Using SELDI method, the pre-, mid- and post-treatment peripheral blood mononuclear cell (PBMC) protein fingerprint of them was created respectively, which was then managed to screen out the markers by using ZUCI-PDAS package for establishing a diagnosis model.

RESULTS

Study was completed in 15 cases of the treatment group with 2 cases dropped out and 1 case lost. Before treatment, 44 protein peaks in the treatment group were found significantly different to those in the control group (P<0.01), and the sensitivity and specificity of the created models, 10542 Da m/z and 2554 Da m/z, reached 100%. After a 12-week treatment, 30 peaks were found significantly different between the two groups (P<0.01), and the sensitivity of 3365, 7104, 3882 and 6796 Da m/z created peak models was 100%, its specificity being 93.33%. Comparing the 35 samples (pre-, mid- and post-treatment) got from the treatment group with the 15 samples from the control group, significant difference was found in 55 peaks (P<0.01), the sensitivity and specificity of the 7103, 3882, 7143 Da m/z created peak models was 100% and 91.43% respectively.

CONCLUSION

Significant differences of PBMC protein expression patterns were found between SLE patients of YDHS and healthy persons at times of before, during and after treatment, suggesting that SELDI may be used as a new method to create the diagnosis model, and its application in effecter protein screening, activity scoring and Chinese medicine syndrome type researching are expectable and waiting for further study in depth.

摘要

目的

探讨表面增强激光解吸电离飞行时间质谱（SELDI）蛋白质芯片技术在系统性红斑狼疮（SLE）诊断及中医辨证分型研究中的应用。

方法

选取18例轻中度阴虚内热证SLE女性患者作为治疗组，15名健康女性志愿者作为对照组。采用SELDI技术分别检测两组治疗前、治疗中及治疗后外周血单个核细胞（PBMC）的蛋白质指纹图谱，运用ZUCI-PDAS软件包筛选标志物，建立诊断模型。

结果

治疗组完成15例，脱落2例，失访1例。治疗前，治疗组与对照组比较有44个蛋白峰差异有统计学意义（P<0.01），建立的10542 Da m/z和2554 Da m/z诊断模型的敏感度和特异度均达100%。治疗12周后，两组比较有30个蛋白峰差异有统计学意义（P<0.01），建立的3365、7104、3882及6796 Da m/z诊断模型的敏感度为100%，特异度为93.33%。将治疗组35份标本（治疗前、治疗中及治疗后）与对照组15份标本比较，有55个蛋白峰差异有统计学意义（P<0.01），建立的7103、3882、7143 Da m/z诊断模型的敏感度和特异度分别为100%和91.43%。