高通量抗体检测技术用于鉴定和定量辐射响应蛋白生物标志物。
High-throughput antibody-based assays to identify and quantify radiation-responsive protein biomarkers.
机构信息
Center for Radiological Research, College of Physicians & Surgeons, Columbia University, New York, New York 10032, USA.
出版信息
Int J Radiat Biol. 2010 Apr;86(4):321-8. doi: 10.3109/09553000903564034.
PURPOSE
After a radiological 'dirty bomb' incident in a major metropolitan center, substantial numbers of people may be exposed to radiation. However, only a fraction of those individuals will need urgent medical attention. Consequently, a rapid screening test is needed to identify those people who require immediate treatment.
MATERIAL AND METHODS
Ten normal human cell lines were screened by enzyme-linked immunosorbent assay (ELISA) for the expression of a dozen secreted cytokines that have been reported to have changes in protein or mRNA levels at 1, 2, and 3 days after 0-10 Gy irradiation using (137)Cs gamma rays at 0.82 Gy min(-1). After this systematic in vitro screen, we measured changes in the level of a subset of these candidate proteins in plasma from irradiated C57BL/6 mice (n = 3 per group), comparing shams with a single radiation dose (5 Gy X-rays) at 3.7 Gy min(-1) at 6 h after irradiation.
RESULTS
We identified four cytokine molecules that had altered levels after radiation exposure, one of which, Interleukin (IL) 6, was consistently elevated after irradiation in vitro and in vivo.
CONCLUSIONS
Our findings underscore the potential for IL6 as a marker for an immunoassay-based, rapid, high-throughput biodosimeter.
目的
在大都市中心发生放射性“脏弹”事件后，大量人员可能会受到辐射。然而，只有其中一部分人需要紧急医疗护理。因此，需要一种快速筛选测试来识别那些需要立即治疗的人。
材料和方法
通过酶联免疫吸附试验（ELISA），对十种正常人类细胞系进行了筛选，以检测十几种分泌细胞因子的表达情况。这些细胞因子的蛋白或 mRNA 水平在 137Csγ射线（0.82 Gy/min）辐射后 1、2 和 3 天发生了变化。在进行这项系统的体外筛选后，我们测量了照射后的 C57BL/6 小鼠（每组 3 只）血浆中这些候选蛋白亚组的水平变化，比较了假照射组与单次照射组（5 Gy X 射线）的差异，单次照射的剂量率为 3.7 Gy/min，照射后 6 小时进行检测。
结果
我们鉴定出了四种细胞因子分子，它们在辐射暴露后水平发生了改变，其中一种细胞因子白细胞介素 6（IL-6）在体外和体内照射后均持续升高。
结论
我们的发现强调了 IL-6 作为一种基于免疫测定的快速高通量生物剂量计的标记物的潜力。
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