自身免疫性溶血性贫血中的肌动蛋白抗体。
Antibodies to actin in autoimmune haemolytic anaemia.
机构信息
Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, Switzerland.
出版信息
BMC Vet Res. 2010 Mar 30;6:18. doi: 10.1186/1746-6148-6-18.
BACKGROUND
In autoimmune haemolytic anaemia (AIHA), autoreactive antibodies directed against red blood cells are up-regulated, leading to erythrocyte death. Mycoplasma suis infections in pigs induce AIHA of both the warm and cold types. The aim of this study was to identify the target autoantigens of warm autoreactive IgG antibodies. Sera from experimentally M. suis-infected pigs were screened for autoreactivity.
RESULTS
Actin-reactive antibodies were found in the sera of 95% of all animals tested. The reactivity was species-specific, i.e. reactivity with porcine actin was significantly higher than with rabbit actin. Sera of animals previously immunised with the M. suis adhesion protein MSG1 showed reactivity with actin prior to infection with M. suis indicating that molecular mimicry is involved in the specific autoreactive mechanism. A potentially cross-reactive epitope was detected.
CONCLUSIONS
This is the first report of autoreactive anti-actin antibodies involved in the pathogenesis of autoimmune haemolytic anaemia.
背景
在自身免疫性溶血性贫血（AIHA）中，针对红细胞的自身反应性抗体被上调，导致红细胞死亡。猪的猪支原体感染会引起温型和冷型自身免疫性溶血性贫血。本研究旨在确定温型自身反应性 IgG 抗体的靶自身抗原。用实验性猪支原体感染猪的血清筛选自身反应性。
结果
在所有检测的动物中，95%的血清中发现了肌动蛋白反应性抗体。反应具有种属特异性，即与猪肌动蛋白的反应性明显高于与兔肌动蛋白的反应性。先前用猪支原体黏附蛋白 MSG1 免疫的动物的血清在感染猪支原体之前就显示出与肌动蛋白的反应性，表明分子模拟参与了特异性自身反应机制。检测到一个潜在的交叉反应表位。
结论
这是首次报道自身反应性抗肌动蛋白抗体参与自身免疫性溶血性贫血发病机制的研究。
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