来自WP72/27的新型隐蔽质粒pLP2.5 - 11和pLP3.0 - 4的特性鉴定与测序分析
Characterization and sequencing analysis of pLP2.5-11 and pLP3.0-4 novel cryptic plasmids from WP72/27.
作者信息
Nitipan Supachai, Saithong Pramuan
机构信息
Department of Biology, Faculty of Science, Thaksin University, Phattalung Campus, Phattalung, 93210 Thailand.
Microbial Technology for Agriculture, Food and Environment Research Center, Thaksin University, Phatthalung Campus, Phatthalung, 93210 Thailand.
出版信息
3 Biotech. 2023 Aug;13(8):263. doi: 10.1007/s13205-023-03684-y. Epub 2023 Jul 3.
UNLABELLED
We sequenced and described two cryptic plasmids from strain WP72/27, termed pLP2.5-11 (OP831909) and pLP3.0-4 (OP831910). Nucleotide sequencing gave the sizes of pLP2.5-11 and pLP3.0-4 as 2754 and 3197 base pairs, with G + C contents 38.89% and 40.88% and predicted two and eight putative open reading frames, respectively. The RepA protein of pLP2.5-11 shared a 99% identity with pC30il, pLP1 and pC30il, whereas the RepB protein of pLP3.0-4 shared a 98% identity with pXY3, a member of the rolling-circle replication (RCR) pC194 family. The origin of plasmid replication was predicted to consist of inverted and directed repeat sequences upstream of the Rep genes. Sequence analysis predicted that both pLP2.5-11 and pLP3.0-4 plasmids replicate via a rolling-circle process.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13205-023-03684-y.
未标记
我们对菌株WP72/27的两个隐蔽质粒进行了测序和描述，分别命名为pLP2.5 - 11（OP831909）和pLP3.0 - 4（OP831910）。核苷酸测序结果显示，pLP2.5 - 11和pLP3.0 - 4的大小分别为2754和3197个碱基对，G + C含量分别为38.89%和40.88%，预测分别有两个和八个推定的开放阅读框。pLP2.5 - 11的RepA蛋白与pC30il、pLP1和pC30il有99%的同一性，而pLP3.0 - 4的RepB蛋白与滚环复制（RCR）pC194家族成员pXY3有98%的同一性。预计质粒复制起点由Rep基因上游的反向和正向重复序列组成。序列分析预测pLP2.5 - 11和pLP3.0 - 4质粒均通过滚环过程进行复制。
补充信息
在线版本包含可在10.1007/s13205 - 023 - 03684 - y获取的补充材料。
Zotero 插件