Department of Cancer Center, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, PR China.
Biosens Bioelectron. 2010 Jun 15;25(10):2379-83. doi: 10.1016/j.bios.2010.03.009. Epub 2010 Mar 11.
A new, highly sensitive electrochemical immunosensor with a sandwich-type immunoassay format was designed to quantify carcinoembryonic antigen (CEA), as a model tumor marker, using nanogold-enwrapped graphene nanocomposites (NGGNs) as trace labels in clinical immunoassays. The device consisted of a glassy carbon electrode coated with Prussian Blue (PB) on whose surface gold nanoparticles were electrochemically deposited to the further modified with the specific analyte-capturing molecule, anti-CEA antibodies. The immunoassay was performed using horseradish peroxidase (HRP)-conjugated anti-CEA as secondary antibodies attached on the NGGN surface (HRP-anti-CEA-NGGN). The method using HRP-anti-CEA-NGGNs as detection antibodies shows high signal amplification, and exhibits a dynamic working range of 0.05-350 ng/mL with a low detection limit of 0.01 ng/mL CEA (at 3s). The assayed results of serum samples with the sensor received an acceptable agreement with the reference values. Importantly, the methodology provides a promising ultrasensitive assay strategy for clinical applications.
一种新型的、高灵敏度的电化学免疫传感器，采用三明治型免疫分析形式，使用纳米金包裹的石墨烯纳米复合材料（NGGNs）作为示踪标记物，用于临床免疫分析中对癌胚抗原（CEA）的定量检测，作为肿瘤标志物的模型。该装置由玻碳电极组成，表面涂有普鲁士蓝（PB），在其表面电化学沉积金纳米颗粒，进一步修饰与特定分析物捕获分子（抗 CEA 抗体）。免疫分析采用辣根过氧化物酶（HRP）标记的抗 CEA 作为次级抗体，附着在 NGGN 表面（HRP-抗 CEA-NGGN）。该方法使用 HRP-抗 CEA-NGGN 作为检测抗体，具有较高的信号放大作用，具有 0.05-350ng/mL 的动态工作范围，CEA 的检测限低至 0.01ng/mL（3s）。该传感器对血清样本的检测结果与参考值具有良好的一致性。重要的是，该方法为临床应用提供了一种有前途的超灵敏分析策略。
