Plant & Food Research Te Puke, 412 No 1 Road, RD 2, Te Puke 3182, New Zealand.
J Sci Food Agric. 2010 Apr 30;90(6):1071-80. doi: 10.1002/jsfa.3924.
Many deciduous, perennial fruit crops require winter chilling for adequate budbreak and flowering. Recent research has shown that changes in sugar and amino acid profiles are associated with the release of buds from dormancy. This paper uses FTIR spectrometry to provide an alternative mechanism for tracking metabolic changes in the meristems of kiwifruit buds during winter dormancy. The results suggest that the application of multivariate analysis to FTIR spectra has the potential to be a reliable and fast method for detecting structural and compositional changes in fruit crops.
Ten wave numbers of the FTIR spectra are used to calculate a bud development function. This function has been validated using data from two seasons and four orchards, and by monitoring the effects of hydrogen cyanamide application, sugar concentrations and soil temperatures on this function. These wave numbers appear to be associated with carbohydrate, pectin and cellulose levels in the meristems.
It is expected that this FTIR signature can be used to advance our understanding of the influence of the various environmental and physiological factors on the breaking of bud dormancy and shoot outgrowth, including the optimum timing and concentrations of applications of budbreak regulators, such as hydrogen cyanamide.
许多落叶和多年生水果作物需要冬季寒冷才能充分打破休眠和开花。最近的研究表明，糖和氨基酸谱的变化与芽从休眠中释放有关。本文使用傅里叶变换红外（FTIR）光谱法提供了一种替代机制，用于跟踪猕猴桃芽在冬季休眠期间的分生组织中的代谢变化。结果表明，多元分析在 FTIR 光谱中的应用有可能成为检测水果作物结构和组成变化的可靠和快速方法。
使用 FTIR 光谱的十个波数来计算芽发育函数。该函数已使用两个季节和四个果园的数据进行了验证，并通过监测氢氰酸应用、糖浓度和土壤温度对该函数的影响来验证。这些波数似乎与分生组织中的碳水化合物、果胶和纤维素水平有关。
预计这种 FTIR 特征可用于深入了解各种环境和生理因素对打破休眠和芽生长的影响，包括打破休眠调节剂（如氢氰酸）的最佳应用时间和浓度。
