Choe B K, Pontes E J, Rose N R, Henderson M D
Invest Urol. 1978 Jan;15(4):312-8.
The immunologic specificity of human prostatic acidphosphatase has been established by several previous investigations as well as in this study. An apparent exception to this specificity was observed--a case of pancreatic islet cell carcinoma metastasized to the liver produced acid phosphatase that was immunologically indistinguishable from the prostatic acid phosphatase. In this case, the possibility of prostatic involvement was convincingly ruled out by clinical follow-ups and by postmortem pathologic studies. Highly purified prostatic acid phosphatase and this tumor acid phosphatase exhibited very similar Km values and identical molecular weights. Immunochemical analysis of the two enzymes using antiprostatic acid phosphatase sera showed that enzymes are antigenically identical. The implications of our observation are discussed in relation to clinical application of immunoassays for prostatic phosphatase in the future and to the molecular basis of human acid phosphatase polymorphism.
人前列腺酸性磷酸酶的免疫特异性已由先前的多项研究以及本研究确定。观察到这一特异性存在一个明显的例外情况——一例转移至肝脏的胰岛细胞癌产生的酸性磷酸酶在免疫学上与前列腺酸性磷酸酶无法区分。在此病例中，临床随访和尸检病理研究令人信服地排除了前列腺受累的可能性。高度纯化的前列腺酸性磷酸酶和这种肿瘤酸性磷酸酶表现出非常相似的米氏常数（Km值）和相同的分子量。使用抗前列腺酸性磷酸酶血清对这两种酶进行免疫化学分析表明，这两种酶在抗原性上是相同的。我们结合未来前列腺磷酸酶免疫测定的临床应用以及人酸性磷酸酶多态性的分子基础对我们的观察结果的意义进行了讨论。
