Bonefeld-Jørgensen Eva C, Long Manhai
Unit of Cellular & Molecular Toxicology, Centre of Arctic Environmental Medicine, Institute of Public Health, University of Aarhus, 8000 Aarhus, Denmark.
Int J Circumpolar Health. 2010 Apr;69(2):181-94. doi: 10.3402/ijch.v69i2.17434. Epub 2010 Mar 29.
(1) To determine whether plasma dioxin-like (DL) bioactivity differs between Inuit living in different Greenlandic districts, (2) to compare the DL activity of the Inuit having high burdens of POPs with a low-burden study group (Danish volunteers), and (3) to evaluate DL activity associations to POP exposure biomarkers and/or lifestyle factors.
The study was a cross-sectional survey, including randomized inhabitants (70) from 6 different Greenlandic districts and young Danish volunteers (22).
POPs and fatty acids profiles were analysed. Aryl hydrocarbon receptor (AhR) mediated DL-activity was determined by exposure of Hepa1.12cR AhR-CALUX reporter cell line to hexane: ethanol plasma extracts.
The sum PCBs/POPs level of Inuit was more than 10 times higher than the levels found in Danish volunteers, and for both study groups the level was positively associated with age. The TCDD equivalent of the determined DL-activity, AhR-TEQ, differed between the Greenlandic districts. The AhR-TEQ data of the combined Inuit was significantly higher compared with the Danish women. AhR-TEQ of Inuit were positively associated with plasma POPs after adjustment for age and/or the ratio of n-3 to n-6 fatty acids, whereas no correlations were found for the Danish samples.
AhR-TEQ differs between Inuit and Danish plasma samples. Plasma POP levels alone cannot be used as a biomarker for DL-activity. We suggest that the profile and level of plasma POPs, geographical location and diet have the greatest impact on plasma dioxin activity. Further studies are needed to elucidate the differences in geographical determinants of blood DL-activity.
（1）确定居住在格陵兰不同地区的因纽特人血浆中二噁英样（DL）生物活性是否存在差异；（2）比较多氯联苯（POPs）高负荷因纽特人与低负荷研究组（丹麦志愿者）的DL活性；（3）评估DL活性与POP暴露生物标志物和/或生活方式因素之间的关联。
本研究为横断面调查，纳入了来自格陵兰6个不同地区的随机居民（70名）和丹麦年轻志愿者（22名）。
分析了POPs和脂肪酸谱。通过将Hepa1.12cR AhR-CALUX报告细胞系暴露于己烷：乙醇血浆提取物中来测定芳烃受体（AhR）介导的DL活性。
因纽特人的多氯联苯/ POPs总量水平比丹麦志愿者高出10倍以上，且两个研究组的该水平均与年龄呈正相关。所测定的DL活性的2,3,7,8-四氯二苯并对二噁英当量（AhR-TEQ）在格陵兰不同地区之间存在差异。合并后的因纽特人的AhR-TEQ数据显著高于丹麦女性。调整年龄和/或n-3与n-6脂肪酸比例后，因纽特人的AhR-TEQ与血浆POPs呈正相关，而丹麦样本未发现相关性。
因纽特人和丹麦人血浆样本中的AhR-TEQ存在差异。仅血浆POP水平不能用作DL活性的生物标志物。我们认为血浆POPs的谱和水平、地理位置和饮食对血浆二噁英活性影响最大。需要进一步研究以阐明血液DL活性地理决定因素的差异。
