Hacettepe University, Food Engineering Department, 06800, Beytepe, Ankara, Turkey.
Int J Food Microbiol. 2010 May 15;139(3):202-5. doi: 10.1016/j.ijfoodmicro.2010.03.006. Epub 2010 Mar 16.
Aim of the present study was to investigate the detoxification of aflatoxin B(1) and patulin from aqueous solution by probiotic culture of Enterococcus faecium M74 and commercial culture of E. faecium EF031. The effect of the bacterial viability, incubation time and pH of the medium on the binding ability was tested. Also, binding stability was determined by washing the bacteria-mycotoxin complexes with phosphate buffer saline. Both M74 and EF031 strains have the ability to remove aflatoxin B(1) and patulin. While M74 removes 19.3 to 30.5% of aflatoxin B(1) and 15.8 to 41.6% of patulin, EF031 removes 23.4 to 37.5% of aflatoxin B(1) and 19.5 to 45.3% of patulin throughout a 48 h incubation period. The removal of aflatoxin B(1) and patulin was highest at pH 7.0 and 4.0, respectively. The stability of the aflatoxin B(1) and patulin complexes formed with the bacterial strains was found to be high. The viability of the bacteria did not have any significant effect on the detoxification of aflatoxin B(1) and patulin. Detoxification properties of E. faecium could represent new strategies for a possible application in the human diet and animal feed.
本研究旨在探讨屎肠球菌 M74 益生菌培养物和商业屎肠球菌 EF031 对水溶液中黄曲霉毒素 B(1)和棒曲霉素的解毒作用。测试了细菌活力、孵育时间和培养基 pH 对结合能力的影响。此外，通过用磷酸盐缓冲盐水洗涤细菌-真菌毒素复合物来确定结合稳定性。M74 和 EF031 菌株均具有去除黄曲霉毒素 B(1)和棒曲霉素的能力。M74 可去除 19.3%至 30.5%的黄曲霉毒素 B(1)和 15.8%至 41.6%的棒曲霉素，EF031 可去除 23.4%至 37.5%的黄曲霉毒素 B(1)和 19.5%至 45.3%的棒曲霉素在 48 小时的孵育期内。黄曲霉毒素 B(1)和棒曲霉素的去除率在 pH 7.0 和 4.0 时最高。发现与细菌形成的黄曲霉毒素 B(1)和棒曲霉素复合物的稳定性很高。细菌的活力对黄曲霉毒素 B(1)和棒曲霉素的解毒作用没有任何显著影响。屎肠球菌的解毒特性可能代表着在人类饮食和动物饲料中可能应用的新策略。
