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HB2-2对黄曲霉毒素B的生物解毒作用。

Biological Detoxification of Aflatoxin B by HB2-2.

作者信息

Feng Jiangtao, Cao Ling, Du Xiaoyan, Zhang Yvying, Cong Yanxia, He Junbo, Zhang Weinong

机构信息

Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, China.

Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China.

出版信息

Foods. 2024 Jun 15;13(12):1887. doi: 10.3390/foods13121887.

DOI:10.3390/foods13121887
PMID:38928828
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11202875/
Abstract

Aflatoxin B (AFB) contamination in food and feed is a global health and economic threat, necessitating the immediate development of effective strategies to mitigate its negative effects. This study focuses on the isolation and characterization of HB2-2 ( HB2-2) as a potent AFB-degrading microorganism, using morphological observation, biochemical profiling, and 16S rRNA sequence analysis. An incubation of HB2-2 at 32 °C for 96 h in a pH 10 nutrient broth (NB) medium resulted in a remarkable degradation rate of 90.0% for AFB. Furthermore, HB2-2 demonstrated 82.9% AFB degradation rate in the peanut meal, reducing AFB levels from 105.1 to 17.9 μg/kg. The AFB degradation ability of HB2-2 was found to be dependent on the fermentation supernatant. The products of AFB degradation by HB2-2 were analyzed by liquid chromatography-mass spectrometry (LC-MS), and a possible degradation mechanism was proposed based on the identified degradation products. Additionally, cytotoxicity assays revealed a significant reduction in the toxicity of the degradation products compared to the parent AFB. These findings highlight the potential of HB2-2 as a safe and effective method for mitigating AFB contamination in food and feed.

摘要

食品和饲料中的黄曲霉毒素B（AFB）污染是对全球健康和经济的一种威胁，因此需要立即制定有效的策略来减轻其负面影响。本研究聚焦于分离和鉴定HB2-2作为一种高效的AFB降解微生物，采用了形态学观察、生化分析和16S rRNA序列分析。HB2-2在pH值为10的营养肉汤（NB）培养基中于32°C培养96小时，对AFB的降解率高达90.0%。此外，HB2-2在花生粕中对AFB的降解率为82.9%，将AFB水平从105.1μg/kg降至17.9μg/kg。发现HB2-2的AFB降解能力依赖于发酵上清液。通过液相色谱-质谱联用（LC-MS）分析了HB2-2对AFB的降解产物，并根据鉴定出的降解产物提出了可能的降解机制。此外，细胞毒性试验表明，与母体AFB相比，降解产物的毒性显著降低。这些发现突出了HB2-2作为减轻食品和饲料中AFB污染的安全有效方法的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d36d/11202875/4028f5244992/foods-13-01887-g011.jpg
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Mechanisms by which microbial enzymes degrade four mycotoxins and application in animal production: A review.微生物酶降解四种霉菌毒素的机制及其在动物生产中的应用：综述
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Progress in the application of Enterococcus faecium in animal husbandry.
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Front Cell Infect Microbiol. 2023 Jul 28;13:1168189. doi: 10.3389/fcimb.2023.1168189. eCollection 2023.
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Degradation mechanism of aflatoxin B1 and aflatoxin G1 by salt tolerant Bacillus albus YUN5 isolated from 'doenjang', a traditional Korean food.从韩国传统食品“大酱”中分离出的耐盐白芽孢杆菌YUN5对黄曲霉毒素B1和黄曲霉毒素G1的降解机制
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Experimental-theoretical study of laccase as a detoxifier of aflatoxins.漆酶作为黄曲霉毒素解毒剂的实验-理论研究。
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