Amir Ebrahimi Niloufar, Salehi Jouzani Gholamreza, Ebrahimi Mohammad Ali
Department of Biotechnology, Faculty of Agriculture, Payam Nour University, Karaj, Iran.
Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Iran J Microbiol. 2022 Apr;14(2):227-237. doi: 10.18502/ijm.v14i2.9192.
Probiotics are added into the food or feed systems and provide beneficial effects to the human or animal health. This study aimed to isolate the gastrointestinal native strains with high probiotic, cholesterol-assimilation and aflatoxin-degradation capabilities from native chickens.
About 70 isolates were isolated from ileum of the Fars province native chickens and were investigated for their probiotic properties.
Of 70 isolates, 10 showed high probiotic capabilities, including survival at acidic conditions (pH up to 2.5), tolerance of 0.5% bile and 6-10% NaCl salts, growth in a wide range of temperature from 15 to 45°C, antagonistic effects against different important bacterial pathogens ( and ) and sensitivity to some important antibiotics. The selected strains had an aggregation time less than 120 min. The 16S-rDNA sequencing showed that the selected strains were highly related to and Finally, the selected strains in this study along with 10 other probiotic strains isolated and characterized in our pervious study were used to evaluate their cholesterol assimilation and aflatoxin B1 degradation capabilities. The potentials of cholesterol assimilation of the selected strains were significantly different (<0.05) and ranged from 2.3% to 99%. The highest content of cholesterol assimilation was obtained in isolates M20 and M4 with more than 98% absorption. Moreover, four strains 43, OR7, M21 and OR9 were able to absorb AFB1 with 58.6%, 52.33%, 47% and 31.6% efficiency respectively.
It could be concluded that the strains 43, M21 and OR7 showed high probiotic potentials for application in the poultry industry.
益生菌被添加到食品或饲料体系中，对人类或动物健康有益。本研究旨在从本地鸡中分离出具有高益生菌活性、胆固醇同化能力和黄曲霉毒素降解能力的胃肠道天然菌株。
从法尔斯省本地鸡的回肠中分离出约70株菌株，并对其益生菌特性进行研究。
在70株分离菌株中，10株表现出高益生菌活性，包括在酸性条件（pH值高达2.5）下存活、耐受0.5%胆汁和6 - 10%氯化钠盐、在15至45°C的广泛温度范围内生长、对不同重要细菌病原体（和）具有拮抗作用以及对一些重要抗生素敏感。所选菌株的聚集时间少于120分钟。16S - rDNA测序表明，所选菌株与和高度相关。最后，本研究中所选菌株与我们之前研究中分离和鉴定的其他10株益生菌菌株一起用于评估它们的胆固醇同化和黄曲霉毒素B1降解能力。所选菌株的胆固醇同化潜力差异显著（<0.05），范围为2.3%至99%。在分离株M20和M4中获得了最高的胆固醇同化含量，吸收率超过98%。此外，四株菌株43、OR7、M21和OR9能够分别以58.6%、52.33%、47%和31.6%的效率吸收AFB1。
可以得出结论，菌株43、M21和OR7在禽类工业应用中显示出高益生菌潜力。
