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用于高灵敏度H₂O₂检测的电化学还原氧化石墨烯上的细菌过氧化物酶

Bacterial Peroxidase on Electrochemically Reduced Graphene Oxide for Highly Sensitive H O Detection.

作者信息

Bhardwaj Sheetal K, Knaus Tanja, Garcia Amanda, Yan Ning, Mutti Francesco G

机构信息

Van't Hoff Institute for Molecular Sciences HIMS-Biocat & HetCat, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands.

出版信息

Chembiochem. 2022 Sep 5;23(17):e202200346. doi: 10.1002/cbic.202200346. Epub 2022 Jul 20.

DOI:10.1002/cbic.202200346
PMID:35723909
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9543142/
Abstract

Peroxidase enzymes enable the construction of electrochemical sensors for highly sensitive and selective quantitative detection of various molecules, pathogens and diseases. Herein, we describe the immobilization of a peroxidase from Bacillus s. (BsDyP) on electrochemically reduced graphene oxide (ERGO) deposited on indium tin oxide (ITO) and polyethylene terephthalate (PET) layers. XRD, SEM, AFM, FT-IR and Raman characterization of the sensor confirmed its structural integrity and a higher enzyme surface occupancy. The BsDyP-ERGO/ITO/PET electrode performed better than other horseradish peroxidase-based electrodes, as evinced by an improved electrochemical response in the nanomolar range (linearity 0.05-280 μM of H O , LOD 32 nM). The bioelectrode was mechanically robust, active in the 3.5-6 pH range and exhibited no loss of activity upon storage for 8 weeks at 4 °C.

摘要

过氧化物酶能够构建用于高灵敏度和选择性定量检测各种分子、病原体和疾病的电化学传感器。在此，我们描述了将来自芽孢杆菌属的一种过氧化物酶（BsDyP）固定在沉积于氧化铟锡（ITO）和聚对苯二甲酸乙二酯（PET）层上的电化学还原氧化石墨烯（ERGO）上。传感器的X射线衍射（XRD）、扫描电子显微镜（SEM）、原子力显微镜（AFM）、傅里叶变换红外光谱（FT-IR）和拉曼光谱表征证实了其结构完整性和更高的酶表面占有率。BsDyP-ERGO/ITO/PET电极的性能优于其他基于辣根过氧化物酶的电极，在纳摩尔范围内具有改善的电化学响应（H₂O₂线性范围为0.05 - 280 μM，检测限为32 nM）证明了这一点。该生物电极机械性能稳健，在3.5 - 6的pH范围内具有活性，并且在4℃下储存8周后活性没有损失。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/2dd42c9e3165/CBIC-23-0-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/b27fea11ca60/CBIC-23-0-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/2b84cddecc47/CBIC-23-0-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/21bb5b23d1cc/CBIC-23-0-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/e2cdb694c316/CBIC-23-0-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/3b406e01867f/CBIC-23-0-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/2dd42c9e3165/CBIC-23-0-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/b27fea11ca60/CBIC-23-0-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/2b84cddecc47/CBIC-23-0-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/21bb5b23d1cc/CBIC-23-0-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/e2cdb694c316/CBIC-23-0-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/3b406e01867f/CBIC-23-0-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca0/9543142/2dd42c9e3165/CBIC-23-0-g006.jpg

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