Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu, PR China.
J Cardiothorac Vasc Anesth. 2010 Feb;24(1):99-103. doi: 10.1053/j.jvca.2009.10.016.
The purpose of this study was to investigate whether adding emulsified isoflurane to St Thomas cardioplegia solution could enhance the cardiac protection after cardioplegic arrest in rats.
A randomized, blind study.
A university laboratory.
Thirty male Sprague-Dawley rats.
Thirty isolated heart preparations were randomly divided into 3 groups (n = 10/group) according to the different cardioplegia solutions being given: St Thomas solution mixed with emulsified isoflurane (containing 2.8% of isoflurane, group EI), St Thomas solution mixed with emulsified Intralipid (Huarui Pharmacy, Wuxi, Jiangsu, China) (group EL), and St Thomas solution alone (group St). In the 35-minute normothermic ischemia period, infusion of cardioplegia solution was repeated every 15 minutes. After the 35-minute ischemia period, the heart was perfused with Krebs-Henseleit buffer for another 2 hours.
The functional parameters of the heart were monitored throughout the experiments. The coronary effluent was collected for measuring the activity of CK-MB 30 minutes after reperfusion, and the infarct size was assessed at the end of reperfusion. The infarct size in group EI (24% +/- 4%) was reduced when compared with that in group EL (31% +/- 8%, p < 0.05) and group St (43% +/- 9%, p < 0.001). The CK-MB activity in group EI was decreased significantly when compared with that in group EL and group St (p < 0.05). The functional recovery in group EI also was improved. Compared with standard St Thomas solution alone, adding 30% Intralipid alone also significantly reduced the infarct size and the CK-MB leakage and improved the recovery of the mechanical function.
St Thomas cardioplegia solution supplemented with emulsified isoflurane enhanced its cardioprotection in an isolated heart ischemia reperfusion injury model in rats.
本研究旨在探讨在停搏液中添加乳化异氟醚是否能增强大鼠心脏停搏后的心肌保护作用。
随机、盲法研究。
一所大学实验室。
30 只雄性 Sprague-Dawley 大鼠。
根据使用的不同停搏液，将 30 个分离的心脏标本随机分为 3 组（n = 10/组）：含 2.8%异氟醚的 Thomas 停搏液与乳化异氟醚混合组（EI 组）、Thomas 停搏液与乳化 Intralipid（江苏无锡华瑞制药有限公司）混合组（EL 组）和 Thomas 停搏液单独使用组（St 组）。在 35 分钟的常温缺血期内，每 15 分钟重复输注停搏液。35 分钟缺血期后，用 Krebs-Henseleit 缓冲液再灌注 2 小时。
整个实验过程中监测心脏的功能参数。再灌注 30 分钟后收集冠状流出液，测定 CK-MB 活性，并在再灌注结束时评估梗死面积。与 EL 组（31% +/- 8%）和 St 组（43% +/- 9%）相比，EI 组（24% +/- 4%）的梗死面积减小（p < 0.05）。与 EL 组和 St 组相比，EI 组 CK-MB 活性明显降低（p < 0.05）。EI 组的功能恢复也得到改善。与单独使用标准 Thomas 停搏液相比，单独添加 30% Intralipid 也显著减少了梗死面积和 CK-MB 漏出，并改善了机械功能的恢复。
在大鼠离体心脏缺血再灌注损伤模型中，Thomas 停搏液中添加乳化异氟醚增强了其心肌保护作用。
