Central Laboratories Unit, Qatar University, Doha, Qatar.
Biol Trace Elem Res. 2011 Apr;140(1):103-13. doi: 10.1007/s12011-010-8677-2. Epub 2010 Apr 1.
The determinations of selenium isotopes in biological samples were performed using both inductively coupled plasma collision/reaction cell quadruple mass spectrometer (CRC-ICP-QMS) and inductively coupled plasma sector field mass spectrometers (SF-ICP-MS). To significantly decrease the argon-based interferences at m/z 74 ((36)Ar(38)Ar), 76 ((38)Ar(38)Ar, (40)Ar(36)Ar), 78 ((38)Ar(40)Ar), and 80 ((40)Ar(40)Ar), the gas-flow rates of a helium and hydrogen mixture used in the collision cell were optimized to 1.0 mL/min H(2) and 3.5 mL/min He. Under the optimized condition, the precisions for natural selenium isotope ratio measurements of both instruments were evaluated and compared using 100 ppb Se standard solution. A modified external calibration quantification method was applied for the simultaneous determination of clinically used enriched selinocompounds ((77)Se-selenate, (82)Se-selenite, (76)Se-methylseleninic acid(IV), (78)Se-methylselenonic acid(VI)) and to examine their fate in rat organs (liver, kidney, and lung).
使用电感耦合等离子体质谱碰撞/反应池四极杆质谱仪（CRC-ICP-QMS）和电感耦合等离子体质谱扇形场质谱仪（SF-ICP-MS）对生物样品中的硒同位素进行了测定。为了显著降低 m/z 74（(36)Ar(38)Ar）、76（(38)Ar(38)Ar、(40)Ar(36)Ar）、78（(38)Ar(40)Ar）和 80（(40)Ar(40)Ar）处的基于氩的干扰，优化了碰撞池中氦气和氢气混合物的气体流速至 1.0 mL/min H2 和 3.5 mL/min He。在优化条件下，使用 100 ppb Se 标准溶液评估并比较了两种仪器对天然硒同位素比值测量的精密度。应用改进的外部校准定量方法同时测定了临床使用的富硒化合物（(77)Se-硒酸盐、(82)Se-亚硒酸盐、(76)Se-甲基硒代亚氨酸(IV)、(78)Se-甲基硒代戊二酸(VI))，并研究了它们在大鼠器官（肝脏、肾脏和肺）中的命运。
