纳马勒瓦细胞产生人淋巴母细胞干扰素。
Production of human lymphoblastoid interferon by Namalva cells.
作者信息
Zoon K C, Buckler C E, Bridgen P J, Gurari-Rotman D
出版信息
J Clin Microbiol. 1978 Jan;7(1):44-51. doi: 10.1128/jcm.7.1.44-51.1978.
Abstract
Optimum conditions for growth and interferon production by a human lymphoblastoid cell line, Namalva, have been studied. Adaptation to large-scale production is possible utilizing either Sendai virus or Newcastle disease virus. Priming of cultures before induction is unnecessary. The interferon produced has properties similar to human leukocyte interferon. The production of lymphoblastoid interferon per cell is increased two- to fourfold after dilution with serum-free medium of a saturation-density culture of Namalva induced with Newcastle disease virus. Maximum interferon yields were obtained 27 h after the addition of virus, using cultures diluted to 4 X 10(5) to 9 X 10(5) cells per ml. The presence of glutamine in the dilution medium was required for maximum interferon production. Newcastle disease virus appeared to inhibit the rates of RNA and protein synthesis more effectively in the diluted cultures.
摘要
已对人淋巴母细胞系Namalva生长和产生干扰素的最佳条件进行了研究。利用仙台病毒或新城疫病毒都有可能适应大规模生产。诱导前对培养物进行预刺激没有必要。所产生的干扰素具有与人白细胞干扰素相似的特性。用新城疫病毒诱导的Namalva饱和密度培养物，用无血清培养基稀释后，每细胞产生的淋巴母细胞干扰素增加了2至4倍。在加入病毒后27小时，使用稀释至每毫升4×10⁵至9×10⁵个细胞的培养物可获得最大干扰素产量。稀释培养基中谷氨酰胺的存在是产生最大量干扰素所必需的。新城疫病毒似乎在稀释培养物中更有效地抑制RNA和蛋白质合成的速率。
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Control of specific gene expression in higher organisms. Expression of mammalian genes may be controlled by repressors acting on the translation of messenger RNA.高等生物中特定基因表达的调控。哺乳动物基因的表达可能受作用于信使核糖核酸翻译的阻遏物调控。
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