Pickering L A, Kronenberg L H, Stewart W E
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5938-42. doi: 10.1073/pnas.77.10.5938.
Several established lines of human lymphoblastoid cells were evaluated for abilities to produce interferons. Some cell lines were able to produce interferon when induced with either Newcastle disease virus or Sendai virus, whereas others failed to produce detectable interferon when so induced. However, several cell lines were able to spontaneously produce interferon without induction. Spontaneously produced interferon was liberated by cells only during logarithmic growth phase, reaching levels ranging from about 10 reference units/ml of growth medium for some cell lines to 1000 reference units/ml for others. The interferons produced by induced lymphoblastoid cells and the spontaneously produced interferons were all characterized as type I human leukocyte interferon by high levels of cross-species antiviral activities on bovine cells and by neutralizations by antiserum to human leukocyte interferon but not by antiserum to human fibroblast interferon. However, analysis by electrophoresis in sodium dodecyl sulfate/polyacrylamide gels revealed that spontaneously produced interferon was less size heterogeneous than human leukocyte interferon, migrating as a single band of activity with a peak at 20,000 daltons, whereas human leukocyte interferon contained peaks of major activity at 23,000 and 18,000 daltons and virus-induced Namalva lymphoblastoid cell interferon migrated predominantly as the 18,000-dalton form. Also, although neither virus-induced primary leukocyte interferon nor any of the virus-induced lymphoblastoid cell interferons were neutralized by antiserum to mouse interferon, all of the spontaneously produced interferons were neutralized by antiserum to mouse interferons. These data suggest significant structural similarities between the active cores of certain interferons from phylogenetically diverse animal species.
对几种已建立的人类淋巴母细胞系产生干扰素的能力进行了评估。一些细胞系在用新城疫病毒或仙台病毒诱导时能够产生干扰素，而其他细胞系在如此诱导时则未能产生可检测到的干扰素。然而，有几种细胞系能够在未诱导的情况下自发产生干扰素。自发产生的干扰素仅在对数生长期由细胞释放，对于某些细胞系，其水平范围为每毫升生长培养基约10个参考单位，而对于其他细胞系则为每毫升1000个参考单位。诱导的淋巴母细胞系产生的干扰素和自发产生的干扰素，通过对牛细胞具有高水平的跨物种抗病毒活性以及被抗人白细胞干扰素血清中和但不被抗人成纤维细胞干扰素血清中和，均被鉴定为I型人白细胞干扰素。然而，通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳分析发现，自发产生的干扰素在大小上的异质性低于人白细胞干扰素，以单一活性带迁移，峰值在20,000道尔顿，而人白细胞干扰素在23,000和18,000道尔顿处有主要活性峰，病毒诱导的Namalva淋巴母细胞系干扰素主要以18,000道尔顿的形式迁移。此外，虽然病毒诱导的原代白细胞干扰素和任何病毒诱导的淋巴母细胞系干扰素均未被抗小鼠干扰素血清中和，但所有自发产生的干扰素均被抗小鼠干扰素血清中和。这些数据表明，来自系统发育上不同动物物种的某些干扰素的活性核心之间存在显著的结构相似性。
