Department of Biotechnology and Environmental Sciences, Thapar University, Patiala, 147004 Punjab, India.
Mol Cell Biochem. 2010 Aug;341(1-2):217-23. doi: 10.1007/s11010-010-0452-3. Epub 2010 Apr 4.
The rising incidents of invasive infections due to multidrug resistant Staphylococcus aureus necessitate the exploration of newer targets for development of antibiotics. Pathogenicity of S. aureus is attributed to a wide range of virulence factors. The aim of this study was to screen the production of three virulence factors viz. extracellular protease, extracellular lipase and superoxide dismutase in human pathogenic strains of S. aureus for development of a test panel which could aid in screening of natural products of plant and microbial origin. 27 clinical isolates were compared for their enzyme expression profiles of which eight were finally selected. Sau G5 was the only protease producing organism selected in the test panel, while Sau G3 and Sau G9 were best SOD producers and Sau G16, Sau G18, Sau G22, Sau A5 and Sau A2 exhibited highest expression among different groups of clinical staphylococci.
由于耐多药金黄色葡萄球菌引起的侵袭性感染事件不断增加，因此需要探索新的抗生素开发目标。金黄色葡萄球菌的致病性归因于广泛的毒力因子。本研究旨在筛选三种毒力因子（即细胞外蛋白酶、细胞外脂肪酶和超氧化物歧化酶）在人类致病性金黄色葡萄球菌菌株中的产生情况，以开发一个测试面板，该面板可用于筛选植物和微生物来源的天然产物。对 27 株临床分离株进行了酶表达谱比较，最终选择了 8 株。在测试面板中，只有 Sau G5 是唯一产生蛋白酶的菌株，而 Sau G3 和 Sau G9 是最佳的 SOD 产生菌，Sau G16、Sau G18、Sau G22、Sau A5 和 Sau A2 在不同组的临床葡萄球菌中表现出最高的表达水平。
