Zdzalik Michal, Karim Abdulkarim Y, Wolski Krzysztof, Buda Pawel, Wojcik Kinga, Brueggemann Sarah, Wojciechowski Piotr, Eick Sigrun, Calander Ann-Marie, Jonsson Ing-Marie, Kubica Malgorzata, Polakowska Klaudia, Miedzobrodzki Jacek, Wladyka Benedykt, Potempa Jan, Dubin Grzegorz
Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
FEMS Immunol Med Microbiol. 2012 Nov;66(2):220-9. doi: 10.1111/j.1574-695X.2012.01005.x. Epub 2012 Jul 31.
Proteases of Staphylococcus aureus have long been considered to function as important virulence factors, although direct evidence of the role of particular enzymes remains incomplete and elusive. Here, we sought to provide a collective view of the prevalence of extracellular protease genes in genomes of commensal and pathogenic strains of S. aureus and their expression in the course of human and mouse infection. Data on V8 protease, staphopains A and B, aureolysin, and the recently described and poorly characterized group of six Spl proteases are provided. A phylogenetically diverse collection of 167 clinical isolates was analyzed, resulting in the comprehensive genetic survey of the prevalence of protease-encoding genes. No correlation between identified gene patterns with specific infections was established. Humoral response against the proteases of interest was examined in the sera derived from human patients and from a model mouse infection. The analysis suggests that at least some, if not all, tested proteases are expressed and secreted during the course of infection. Overall, the results presented in this study support the hypothesis that the secretory proteases as a group may contribute to the virulence of S. aureus.
长期以来，金黄色葡萄球菌的蛋白酶一直被认为是重要的毒力因子，尽管特定酶作用的直接证据仍然不完整且难以捉摸。在此，我们试图全面了解共生和致病金黄色葡萄球菌菌株基因组中细胞外蛋白酶基因的普遍性及其在人类和小鼠感染过程中的表达情况。我们提供了关于V8蛋白酶、葡萄溶素A和B、金黄色葡萄球菌溶血素以及最近描述但特征了解较少的六种Spl蛋白酶的数据。我们分析了167株临床分离株，这些分离株在系统发育上具有多样性，从而对蛋白酶编码基因的普遍性进行了全面的基因调查。我们并未发现已鉴定的基因模式与特定感染之间存在相关性。我们检测了来自人类患者和模型小鼠感染血清中针对相关蛋白酶的体液免疫反应。分析表明，在感染过程中，至少部分（如果不是全部）所检测的蛋白酶会表达并分泌。总体而言，本研究结果支持这样一种假说，即作为一个整体的分泌型蛋白酶可能对金黄色葡萄球菌的毒力有贡献。
