Lettry Vivien, Sumie Yasuharu, Mitsuda Kenta, Tagami Masaaki, Hosoya Kenji, Takagi Satoshi, Okumura Masahiro
Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Jpn J Vet Res. 2010 Feb;57(4):197-206.
The objective of this study was to investigate the changes in synovial fluid concentration of collagen type II cleavage site (C2C) and procollagen II C-propeptide (CPII), markers of joint cartilage degeneration and synthesis, respectively, in horses with intraarticular fracture or osteochondrosis dissecans (OCD), and to examine the relationship between arthroscopic findings and these biomarker levels. Synovial fluid was collected from 36 joints in 18 horses (6 fractures and 12 OCDs). Samples from contralateral normal joints, when available, served as controls (n = 12). Concentrations of C2C and CPII were measured using enzyme-linked immunosorbant assays. Moreover, the severity of the cartilage degradation was graded arthroscopically in 16 horses, and the correlation between the C2C and CPII levels and the arthroscopic scores were investigated. Compared to the control, the concentration of C2C was increased in OCD joints but not in fracture joints, whereas the concentration of CPII was increased in fracture joints but not in OCD joints. Within each disease group there was no correlation between biomarker levels and arthroscopic findings. Therefore, although C2C and CPII have diagnostic potential further knowledge is required to provide accurate analysis.
本研究的目的是调查关节内骨折或剥脱性骨软骨炎（OCD）马匹的滑液中II型胶原裂解位点（C2C）和II型前胶原C端前肽（CPII）的浓度变化，这两种物质分别是关节软骨退变和合成的标志物，并研究关节镜检查结果与这些生物标志物水平之间的关系。从18匹马的36个关节（6例骨折和12例OCD）中采集滑液。如有对侧正常关节的样本，则用作对照（n = 12）。使用酶联免疫吸附测定法测量C2C和CPII的浓度。此外，对16匹马进行关节镜检查以评估软骨退变的严重程度，并研究C2C和CPII水平与关节镜评分之间的相关性。与对照组相比，OCD关节中C2C浓度升高，而骨折关节中未升高；而骨折关节中CPII浓度升高，OCD关节中未升高。在每个疾病组中，生物标志物水平与关节镜检查结果之间均无相关性。因此，尽管C2C和CPII具有诊断潜力，但仍需要更多知识以提供准确分析。
