Pucci-Minafra I, Luparello C
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italia.
J Submicrosc Cytol Pathol. 1991 Jan;23(1):67-74.
Different ratios of type V and I collagens were submitted to mixed fibrillogenesis followed by localization of type V collagen within the aggregates by immunoelectron microscopy. At lower concentrations (10-30%), type V collagen segregates into aperiodic filamentous material, peripheral to the cross-banded type I fibrils but making contact in an apparently random manner. Increasing the ratio of type V collagen up to 50% causes the disappearance of collagen fibrils and the formation of a sticky gel composed of weakly immunoreactive long-spacing structures, interspersed with intensely labeled amorphous material. Hybrid type V/type I matrices changed the growth behaviour of 8701-BC carcinoma cells, with inhibition of cell growth being directly related to type V content. This restraining influence on growth was partially reversed when substrates were pre-incubated with low dilutions of anti-type V serum, prior to cell seeding. These findings suggest that the high concentrations of type V collagen, known to exist in vivo in some scirrhous tumors like ductal infiltrating carcinoma of the breast, perturb the normal fibrous architecture of the stroma and concurrently inhibit neoplastic cell growth.
将不同比例的V型和I型胶原蛋白进行混合原纤维形成，随后通过免疫电子显微镜确定V型胶原蛋白在聚集体中的定位。在较低浓度（10%-30%）下，V型胶原蛋白分离成无定形丝状物质，位于交叉带状I型原纤维的外周，但以明显随机的方式接触。将V型胶原蛋白的比例增加至50%会导致胶原原纤维消失，并形成由弱免疫反应性长间距结构组成的粘性凝胶，其间散布着强标记的无定形物质。V型/I型混合基质改变了8701-BC癌细胞的生长行为，细胞生长的抑制与V型含量直接相关。当在细胞接种前将底物用低稀释度的抗V型血清预孵育时，这种对生长的抑制作用部分得到逆转。这些发现表明，已知在体内某些硬癌肿瘤（如乳腺导管浸润癌）中存在的高浓度V型胶原蛋白会扰乱基质的正常纤维结构，并同时抑制肿瘤细胞生长。
