Luparello C, Schillaci R, Pucci-Minafra I, Minafra S
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
Eur J Cancer. 1990 Mar;26(3):231-40. doi: 10.1016/0277-5379(90)90217-h.
Type V collagen is one of the minor components of the extracellular matrix (ECM) whose content is increased in cases of ductal infiltrating carcinomas of the breast. In order to clarify its biological role, we have investigated the effect of this molecule, both as substrate and as soluble factor, on the behaviour of a breast carcinoma cell line (8701-BC) grown in vitro. Cell-collagen adhesion was monitored for 24 h from plating in the absence or presence of serum. The influence of type V collagen on cell growth was followed during 9 days of culture, and the actin-vinculin arrangement was studied by simultaneous fluorescent immuno-staining. The results indicate that type V collagen is not a permissive substrate for neoplastic cell proliferation and dissemination in vitro.
V型胶原蛋白是细胞外基质（ECM）的次要成分之一，在乳腺导管浸润癌病例中其含量会增加。为了阐明其生物学作用，我们研究了这种分子作为底物和可溶性因子对体外培养的乳腺癌细胞系（8701-BC）行为的影响。在有无血清的情况下，从接种开始监测细胞与胶原蛋白的黏附情况，持续24小时。在培养9天期间跟踪V型胶原蛋白对细胞生长的影响，并通过同步荧光免疫染色研究肌动蛋白-纽蛋白的排列。结果表明，V型胶原蛋白在体外不是肿瘤细胞增殖和扩散的允许性底物。
