Rui Jun, Long Dan, Wang Wei, Chou Tao, Zhang Zhi-Guo, Li Xin-Jian, Fang Bing, Yuan Wu, Luo Yong, Zhou Jing
Department of Cardiothoracic Surgery, Chengdu Third People's Hospital, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 Jan;41(1):10-4, 28.
To investigate the effect of valproic acid (VPA) on MICA gene expression in human lung carcinoma cell lines: A549, SP-CA-1, NCIH446.
Different concentration of VPA were applied to treat A549, SP-CA-1, NCIH446 cells respectively and the untreated cells as the control. The effect of the VPA on the cells were observed. Reverse Transcriptase PCR and Immune-Fluorescence staining were used to detect the changes of mRNA, protein level of MICA gene in the three cell lines treated with 1.50 mmol/L and 3.00 mmol/L VPA for 4 days under the concentration of VPA from 0.75 to 12.0 mmol/L. The viability of lung cancer cells after NK effect in the VPA(+) and VPA(-) treatment were evaluated with LDH method.
The inhibition effects were observed when the VPA concentration was 6.0 mmol/L above compared to control group, VPA induced the increasing expression of MICA and in turn enhanced the NK killing effect on the cells in a concentration-depended pattern.
VPA induced MICA expression, NK killing effect in the VPA(+) treatment lung cancer cells was significantly stronger than that of VPA(-) condition, which implied a new mechanism of anticancer method and could be a new approach to cancer therapy.
研究丙戊酸（VPA）对人肺癌细胞系A549、SP-CA-1、NCIH446中MICA基因表达的影响。
分别用不同浓度的VPA处理A549、SP-CA-1、NCIH446细胞，未处理的细胞作为对照。观察VPA对细胞的作用。在0.75至12.0 mmol/L的VPA浓度下，用逆转录聚合酶链反应（RT-PCR）和免疫荧光染色检测1.50 mmol/L和3.00 mmol/L VPA处理4天的三种细胞系中MICA基因的mRNA和蛋白水平变化。用乳酸脱氢酶（LDH）法评估VPA（+）和VPA（-）处理后NK作用下肺癌细胞的活力。
与对照组相比，当VPA浓度高于6.0 mmol/L时观察到抑制作用，VPA诱导MICA表达增加，进而以浓度依赖模式增强NK对细胞的杀伤作用。
VPA诱导MICA表达，VPA（+）处理的肺癌细胞中NK杀伤作用明显强于VPA（-）情况，这暗示了一种新的抗癌方法机制，可能是癌症治疗的一种新途径。
