Shams Shahemabadi Ali, Zavaran Hosseini Ahmad, Shaghasempour Shapour, Masjedi Mohammad Reza, Rayani Majid, Shams Majid, Esphandyari Nasrin, Pouramiri Majid
Department of Immunology, Shaheed Sadughi University of Medical Sciences, Yazd, Iran.
Iran J Immunol. 2010 Mar;7(1):57-63.
Mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tuberculosis is not well known.
The aim of current investigation was to study the M. tuberculosis lipid antigens in tuberculosis pathogenesis.
In the present study M. tuberculosis lipid antigens were extracted. Monocytes and macrophages from multidrug-resistant tuberculosis (MDR-TB), TB patients, asymptomatic healthy individuals with positive tuberculin skin test positive and healthy individuals with negative tuberculin skin test were collected using magnetic cell sorting. The cells were stimulated by M. tuberculosis total lipid antigens and IL-12 and IL-10 in their supernatants were measured by enzyme-linked immunosorbent assay.
The IL-12 production by monocytes in response to M. tuberculosis total sonicate antigens in the MDR-TB patients did not show a considerable difference with the PPD positive healthy subjects, whereas in the active TB patients, IL-12 levels significantly decreased (p<0.05). IL-10 production by monocytes in TB patients in response to total lipid antigens showed a significant increase in comparison to MDR-TB patients and healthy individuals.
In the MDR-TB patients, IL-10 and IL-12 production by monocytes in response to M. tuberculosis lipid antigens are similar to the healthy subjects.
结核分枝杆菌脂质抗原参与该细菌的致病性，但单核细胞/巨噬细胞对这些抗原在结核病中的反应尚不清楚。
本研究旨在探讨结核分枝杆菌脂质抗原在结核病发病机制中的作用。
在本研究中，提取了结核分枝杆菌脂质抗原。使用磁性细胞分选技术收集多药耐药结核病（MDR-TB）患者、结核病患者、结核菌素皮肤试验阳性的无症状健康个体以及结核菌素皮肤试验阴性的健康个体的单核细胞和巨噬细胞。用结核分枝杆菌总脂质抗原刺激细胞，采用酶联免疫吸附测定法检测其上清液中白细胞介素-12（IL-12）和白细胞介素-10（IL-10）的水平。
MDR-TB患者的单核细胞对结核分枝杆菌总超声破碎抗原产生的IL-12与结核菌素阳性健康受试者相比无显著差异，而在活动性结核病患者中，IL-12水平显著降低（p<0.05）。结核病患者的单核细胞对总脂质抗原产生的IL-10与MDR-TB患者和健康个体相比显著增加。
在MDR-TB患者中，单核细胞对结核分枝杆菌脂质抗原产生的IL-10和IL-12与健康受试者相似。
