Department of Clinical Medicine, National Institute for Minamata Disease, Minamata, Kumamoto, Japan.
J Toxicol Sci. 2010 Apr;35(2):217-24. doi: 10.2131/jts.35.217.
To develop an analytical method for methylmercury (MeHg) compounds in biological samples (hair, blood, fish) with reproducibly high recovery of MeHg using heating vaporization atomic absorption spectrometry, we examined the pretreatment process of biological samples such as solubilization, degreasing and solvent extraction of MeHg using the Magos method. Samples were solubilized with NaOH at 70 degrees C and degreased of fat components by chloroform and hexane. Hydrobromic acid (HBr) was used to acidify the solubilized solution to form MeHgBr with high solvent transferability. The fraction containing MeHg-L-cysteine complexes in aqueous solution, which had been reverse-extracted from the toluene layer, were determined as MeHg. Recoveries of MeHgBr were approximately equal 95% with little variation. Analytical values for standards materials for hair and fish using the present method agreed well with certified values.
为了开发一种分析生物样品（头发、血液、鱼类）中甲基汞（MeHg）化合物的方法，我们使用加热蒸发原子吸收光谱法，研究了 Magos 法中生物样品的预处理过程，如 MeHg 的溶解、脱脂和溶剂萃取。样品在 70°C 下用氢氧化钠溶解，并使用氯仿和正己烷去除脂肪成分。氢溴酸（HBr）用于酸化溶解液，形成高溶剂转移能力的 MeHgBr。从甲苯层中反萃取的水溶液中含有 MeHg-L-半胱氨酸配合物的部分被确定为 MeHg。MeHgBr 的回收率约为 95%，变化很小。使用本方法测定头发和鱼类标准物质的分析值与认证值吻合良好。
